摘要
目的:探讨白桦脂醇对慢性不可预知温和应激抑郁(CUMS)模型大鼠的抗抑郁作用,为治疗抑郁症提供基础理论依据。方法:大鼠随机分为正常组、模型组、氟西汀组和白桦脂醇组,采用氟西汀和白桦脂醇干预改进CUMS模型大鼠,通过行为学观察抑郁行为,TUNEL法检测海马神经元凋亡,HPLC-ECD法检测5-羟色胺(5-HT)、去甲肾上腺素(NE)和多巴胺(DA)神经递质水平,免疫印迹检测脑源性神经营养因子(BDNF)、神经营养蛋白3(NT-3)和神经生长因子(NGF)蛋白水平。结果:氟西汀组和白桦脂醇组较模型组大鼠的体质量在3、4周增加,且在4周时白桦脂醇组较氟西汀组的大鼠体质量增加;氟西汀组和白桦脂醇组较模型组大鼠悬尾静止时间、强迫游泳不动时间均延长,且氟西汀组较白桦脂醇组明显延长;氟西汀组和白桦脂醇组较模型组海马凋亡细胞数下降,且白桦脂醇组高于氟西汀组;氟西汀组和白桦脂醇组较模型组大鼠脑组织中5-HT、NE和DA含量均减少,且氟西汀组较白桦脂醇组减少;氟西汀组较模型组大鼠脑组织BDNF、NT-3和NGF蛋白相对表达水平均降低,氟西汀组较白桦脂醇组降低。结论:白桦脂醇能够改善CUMS模型大鼠的抑郁行为,且对其海马神经元具有一定保护作用。
Objective:To investigate the antidepressant effect of betula platyphylla on chronic unpredictable mile stress(CUMS)and depression model rats,and to provide the theoretical basis for the treatment of depression.Methods:Rats were randomly divided into normal group,model group,fluoxetine group and betula platyphylla group.Fluoxetine and betula platyphylla were used to improve CUMS.Depressive behavior and TUNEL neuron apoptosis were detected by behavioral analysis.The level of 5-hydroxytryptamine(5-HT),norepinephrine(NE)and dopamine(DA)neurotransmitters was detected by HPLC-ECD,and brain-derived neurotrophic factor(BDNF),human neurotrophin-3(NT-3)and nerve growth factor(NGF)protein levels were detected by Western blotting.Results:In fluoxetine group and betula platyphylla group,the body weight increased in 3 and 4 weeks,and at week 4,the body weight of betula platyphylla group increased compared with fluoxetine group.The suspension time and forced swimming time in fluoxetine group and betula platyphylla group were longer than those in model group,and they were longer in the fluoxetine group than betula platyphylla group.The number of apoptotic cells in the hippocampus of fluoxetine group and betula platyphylla decreased,and betula platyphylla group was higher than that of fluoxetine group.In fluoxetine group and betula platyphylla group,the contents of 5-HT,NE and DA in brain tissue were all decreased compared with model group,and fluoxetine group was lower than that in Betula platyphylla group.In fluoxetine group and betula platyphylla group,the relative expression levels of BDNF,NT-3 and NGF in brain tissue were all decreased,and fluoxetine group was lower than that in betula platyphylla group.Conclusion:Betula platyphylla can improve depression behavior of CUMS model rats,and has a certain protective effect on hippocampal neurons of CUMS model rats.
作者
傅增辉
金艳
林再红
姜岩
刘晶
杜姝
张广萍
陈团团
Fu Zenghui;Jin Yan;Lin Zaihong;Jiang Yan;Liu Jing;Du Shu;Zhang Guangping;Chen Tuantuan(Department of Neurology,the Third Affiliated Hospital of Qiqihar Medical University,Qiqihar 161002,China)
出处
《解剖学杂志》
CAS
2020年第3期200-205,共6页
Chinese Journal of Anatomy
基金
黑龙江省教育厅基本科研业务费基础研究项目(2017-KYYWF-0700)。
关键词
白桦脂醇
抑郁
凋亡
单胺
神经营养因子
大鼠
betula platyphylla
depression
apoptosis
monoamine
neurotrophic factor
rat
作者简介
第1作者:傅增辉,E-mail:fzh92@foxmail.com。
