摘要
以兰州百合新鲜鳞茎为外植体,选择MS和1/2 MS培养基为基本培养基,采用正交设计研究不同消毒剂组合对外植体存活率、不同植物生长调节剂组合对愈伤组织诱导率和发芽率的影响;并用响应面法优化不同激素组合对生根率的影响。结果表明:(1)最佳消毒剂组合为75%乙醇消毒20 s+2%次氯酸钠溶液浸泡10 min,外植体成活率可达96.30%。(2)愈伤组织诱导最佳培养基配方为MS+0.3 mg·L-1 NAA+3 mg·L-16-BA,诱导率可达96.33%。(3)促进发芽的最佳培养基配方为MS+0.3 mg·L-1 NAA+3 mg·L-16-BA,发芽率可达98.00%。(4)促进生根的最佳培养基配方为1/2 MS+0.5 mg·L-1 NAA+3 mg·L-16-BA+2 mg·L-1活性炭,生根率可达94.00%。本研究建立的组培快繁体系可用于兰州百合的快速大量繁殖,可为兰州百合组培苗的工厂化生产提供参考。
To solve the shortage of Lanzhou lily seedlings,micro-propagation system for mass production of Lanzhou lily seedlings was discussed in this study.Fresh bulbs of Lanzhou lily were used as the initial plant material,and MS and 1/2MS medium were used as the basic medium.The orthogonal design was used to study the effects of different disinfectants on survival rate of explants,and different plant growth regulators on callus and clustered shoots induction.The response surface method also was used to reveal the effects of different hormones combinations on rooting.The results were as follows:(1)The best disinfectant combination was 75%alcohol disinfection for 20 s+2%sodium hypochlorite solution for 10 min.The survival rate of explant was 96.30%.(2)The best medium formulation for callus induction was MS+0.3 mg·L-1 NAA+3 mg·L-16-BA,and the induction rate could reach 96.33%.(3)The best medium formula to promote clustered shoots induction was MS+0.3 mg·L-1 NAA+3 mg·L-16-BA,and the induction rate could reach 98.00%.(4)The best medium formula for rooting was 1/2 MS+0.5 mg·L-1 NAA+3 mg·L-16-BA+2 mg·L-1 activated carbon,the rooting rate could reach 94.00%.
作者
柯义强
郭鹏辉
马洪鑫
杨许花
高丹丹
刘湘君
马忠仁
丁功涛
KE Yiqiang;GUO Penghui;MA Hongxin;YANG Xuhua;GAO Dandan;LIU Xiangjun;MA Zhongren;DING Gongtao(College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730124, China;Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China)
出处
《浙江农业学报》
CSCD
北大核心
2020年第6期1000-1008,共9页
Acta Agriculturae Zhejiangensis
基金
甘肃省国际科技合作项目(17YF1WA166)
科技部援助项目(KY201501005)
“长江学者和创新团队发展计划”创新团队(IRT_17R88)
西北民族大学本科生创新项目(Y18087)
甘肃省科技计划(18JR3RA368)
西北民族大学中央高校基本科研业务费资金(31920190019,31920190202-4)
西北民族大学中央高校基本科研业务费专项资金资助研究生项目(Yxm2020126)。
关键词
兰州百合
快繁体系
植物组培
响应面
Lanzhou lily
rapid propagation system
plant tissue culture
response surface method
作者简介
柯义强(1995—),男,宁夏固原人,硕士研究生,研究方向为食品质量安全与控制,E-mail:184862604@qq.com;通讯作者:高丹丹,E-mail:gaodan0322@163.com。
