摘要
目的探讨多配体蛋白聚糖-1(syndecan-1,SDC-1)在瘢痕疙瘩组织及成纤维细胞中表达情况及其对细胞增殖及迁移的影响。方法采用免疫组织化学方法、qRT-PCR方法及Western blot法检测瘢痕疙瘩组织及成纤维细胞中的SDC-1蛋白表达情况。接下来通过转染SDC-1 siRNA序列,使SDC-1的表达下降。通过CCK-8法检测敲减SDC-1对瘢痕疙瘩成纤维细胞增殖的影响。Western blot法测定敲减SDC-1对迁移相关蛋白MMP-2和MMP-9蛋白表达的影响。结果 SDC-1在瘢痕疙瘩组织及成纤维细胞中呈高表达(P<0.05)。与NC siRNA组相比,SDC-1 siRNA组抑制瘢痕疙瘩成纤维细胞的增殖,同时抑制MMP-9蛋白的表达(P<0.05);而两组间MMP-2蛋白表达差异无统计学意义(P>0.05)。结论 SDC-1在瘢痕疙瘩组织及成纤维细胞中高表达,敲减SDC-1可抑制瘢痕疙瘩成纤维细胞增殖及迁移相关蛋白的表达,这为探讨SDC-1与瘢痕疙瘩成纤维细胞增殖及迁移的相关研究提供了依据。
Objective To investigate the expression of syndecan-1(SDC-1) in keloid tissues and fibroblasts and its effects on cell proliferation and migration.Methods Expression of SDC-1 protein in keloid tissues and fibroblasts was detected by immunohistochemistry,qRT-PCR and Western blot.Then,expression of SDC-1 was decreased by transfection of siRNA SDC-1,and the effect of SDC-1 knockdown on cell proliferation of keloid fibroblasts was detected by CCK-8 assay.Western blot was used to detect the effect of SDC-1 knockdown on the expression of migration-related proteins MMP-2 and MMP-9.Results SDC-1 was significantly overexpressed in both keloid tissues and fibroblasts(P<0.05).Compared with the NC siRNA group,the proliferation of keloid fibroblasts was inhibited,and the expression of MMP-9 was suppressed in the SDC-1 siRNA group(P<0.05).However,the expression of MMP-2 protein did not differ significantly between NC siRNA group and SDC-1 siRNA group(P>0.05).Conclusion SDC-1 is significantly overexpressed in both keloid tissues and fibroblasts.SDC-1 knockdown inhibits the proliferation and migration of keloid fibroblasts,which may provide a basis for the study of relationship between SDC-1 and cell proliferation and migration in keloid fibroblasts.
作者
崔晶
金哲虎
金珊
南美兰
崔婧博
金承龙
CUI Jing;JIN Zhehu;JIN Shan;NAN Meilan;CUI Jingbo;JIN Chenglong(Department of Dermatology,Yanbian University Hospital,Yanji 133000,China)
出处
《中国皮肤性病学杂志》
CAS
CSCD
北大核心
2020年第6期617-621,共5页
The Chinese Journal of Dermatovenereology
基金
国家自然基金资助项目(81960561)
吉林省教育厅(吉教科合字[2016]第270号)。
作者简介
通信作者:金承龙,E-mail:15526770921@163.com。
