摘要
目的:观察梓醇对AAPH损伤条件下的大鼠施万细胞96(RSC96)抗凋亡的影响。方法:通过体外培养RSC96,建立AAPH损伤时间浓度梯度模型:0、40、80、120、160、200mmol/L AAPH对RSC96损伤2、3、4h,选取合适的损伤浓度和时间,给予不同梓醇浓度0、5、10、50、100、150μmol/L进行干预,选取最佳的浓度。光镜观察RSC96细胞形态结构,CCK-8检测细胞活性程度。结果:AAPH损伤时间浓度模型显示浓度为120mmol/L,时间为3h为最佳浓度和时间,经不同浓度梓醇干预后,10μmol/L为最佳梓醇干预浓度。与正常对照组比较,模型组CCK-8吸光度OD值显著降低(P<0.05),细胞突起显著缩短。药物干预组和模型组比较,梓醇能显著提高细胞存活率与贴壁细胞数(P<0.05),吸光度OD值显著增加(P<0.05),细胞突起长度显著增长。结论:梓醇能减轻AAPH对RSC96的损伤,增加RSC96的细胞存活率,然其作用机制有待进一步探索。
Objective:To observe the effects of catalpol on rat schwann cell 96(RSC96)anti-apoptosis under AAPH injury.Methods:RSC96 was cultured in vitro,to establish the model of AAPH injury time and concentration gradient,different the concentration of AAPH of 0,40,80,120,160,200mmol/L damaging RSC96 by the different AAPH injury time of 2,3,4h.Selecting the right time and concentration,use different concentration catalpol of 0,5,10,50,100,150μmol/L to intervene RSC96.To select the best concentration,observe RSC96 cell morphology,CCK-8 used to detect the cell activity.Results:AAPH damage model showed that the concentration of 120mmol/L,time of 3h was optimal concentration and time.By different concentration of catalpol after the intervention,the group of concentration of 10μmol/L was the best,compared with blank control group,damage model group,CCK-8 absorbance OD value significantly reduced(P<0.05),the cell bulge shortened significantly.Intervention of catalpol and damage model group by comparison,catalpol can significantly increase the survival rate and the number of adherent cells(P<0.05),the OD value increasing significantly(P<0.05),bulge growing significantly.Conclusion:Catalpol can reduce the damage of AAPH to RSC96 and increase the cell survival rate of RSC96,but its mechanism needs to be further explored.
作者
李珊
李娜
杨涛
仝延萍
关瑞熙
王昕
郭安臣
樊永平
LI Shan;LI Na;YANG Tao;TONG Yan-ping;GUAN Rui-xi;WANG Xin;GUO An-chen;FAN Yong-ping(Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100700,China;Beijing Integrated Chinese and Western Medicine Research Institution on Encephalopathy,Beijing 100070,China;Department of Traditional Chinese Medicine,Beijing Tiantan Hospital,Capital Medical University,Beijing 100070,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2020年第4期2060-2062,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
北京市自然科学基金-市教委联合项目(No.KZ201910025035)。
作者简介
通讯作者:樊永平,北京市丰台区南四环路119号首都医科大学附属北京天坛医院中医科,邮编:100070,电话:010-59976662,E-mail:yongpingf@hotmail.com。
