摘要
目的探讨亚甲基四氢叶酸还原酶(MTHFR)基因C677T和A1298C位点多态性与台州地区儿童哮喘发生的相关性。方法采用荧光定量PCR技术检测122例哮喘儿童和100例健康儿童MTHFR C677T和A1298C位点的多态性,比较其在2组儿童中的基因型及等位基因频率的分布差异。结果哮喘组儿童MTHFR基因C677T位点3种基因型CC、CT、TT的频率分别为23. 8%、39. 3%和36. 9%,与对照组相比差异有统计学意义(χ~2=7. 356,P <0. 05);哮喘组T等位基因频率(56. 6%)显著高于对照组(43. 0%),其相对于C等位基因的患病危险度(OR)为1. 315 (95%CI:1. 084~1. 597);A1298C位点3种基因型AA、AC、CC的频率分别为63. 9%、32. 0%和4. 1%,C等位基因频率为20. 1%,与对照组相比差异均无统计学意义(P> 0. 05)。结论 MTHFR基因1298位点多态性与台州地区儿童哮喘发生无相关性,677位点C→T变异可能增加本地区儿童发生支气管哮喘的风险。
Objective To investigate the association of methylenetetrahydrofolate reductase(MTHFR)gene C677 T and A1298 C polymorphisms with asthma in children in Taizhou.Methods The polymorphisms of MTHFR C677 T and A1298 C loci in 122 asthmatic children and 100 healthy children were detected by fluorescence quantitative PCR,and the genotypes and allele frequencies of MTHFR C677 T and A1298 C loci were compared between the two groups.Results The frequencies of CC,CT and TT at C677 T locus of MTHFR gene in asthmatic children were 23.8%,39.3%and 36.9%respectively,which were distinctly different from those in control group(χ~2=7.356,P<0.05).The frequencies of T allele in asthmatic children(56.6%)were significantly higher than those in control group(43.0%).The frequencies of AA,AC and CC at A1298 C locus were 63.9%,32.0%and 4.1%respectively,and the frequencies of T allele were 20.1%.Compared with the control group,there was no statistical significance on the difference(P>0.05).Conclusion MTHFR gene 1298 polymorphism is not associated with asthma in Taizhou children.C→T mutation of 677 loci may increase the risk of asthma in children.
作者
陈文举
王攀
钟倩怡
梁玲芝
李招云
CHEN Wen-ju;WANG Pan;ZHONG Qian-yi;LIANG Ling-zhi;LI Zhao-yun(Taizhou Central Hospital(The Afiliated Hospital of Taizhou College),Taizhou,Zhejiang 318000,China)
出处
《中国卫生检验杂志》
CAS
2020年第9期1095-1097,共3页
Chinese Journal of Health Laboratory Technology
作者简介
陈文举(1978-),男,本科,副主任技师,主要从事临床生化与分子生物学检验工作。
