摘要
木质素的脱除是醋糟厌氧消化性能提升的关键。以厌氧发酵后的醋糟为底物,利用黄孢原毛平革菌(Phanerochaete chrysosporium)后处理醋糟脱除木质素的方式,深度提升其产甲烷潜力,并考查不同后处理条件(接种量和后处理时间)下醋糟的降解情况和产甲烷潜力。实验结果表明:加大接种量和延长后处理时间可以促进P.chrysosporium木质纤维素酶的分泌,从而获得更佳的底物降解效果。当接种量为9块、后处理时间为16 d时(实验组T9-16),醋糟的降解效果最佳,总固体和挥发性固体的降解率分别为8.96%和9.91%,纤维素、半纤维素和木质素的降解率分别为7.84%、23.72%和11.84%。进一步的产甲烷潜力实验结果表明,P.chrysosporium后处理有效提高了醋糟的可生物降解性。接种量为6块、后处理时间为16 d的实验组(T6-16)产气最佳,单位质量底物(以挥发性固体计)甲烷产气量达到了246.8 mL·g^-1,是对照组(醋糟未进行后处理)的2.82倍。本研究证实了P.chrysosporium后处理提升醋糟厌氧消化产甲烷潜力的可行性。
Delignification is the key to improve the anaerobic digestion performance of vinegar residue.In this study,vinegar residue after anaerobic fermentation was used as substrate.The delignification was realized by post-treatment of vinegar residue with Phanerochaete chrysosporium,so as to deeply improve the methane production potential.The degradation and methane production potential of vinegar residue were investigated under different post-treatments(inoculation amount and post-treatment time).It was shown that increasing the inoculum amount and prolonging the post-treatment time could promote the secretion of lignocellulase,so as to obtain higher degradation effect.When the inoculation amount was 9 pieces and the post-treatment time was 16 days(experimental group T9-16),the substrate degradation efficiency was the highest.The degradation rates of total solids and violate solids were 8.96%and 9.91%,respectively.And the degradation rates of cellulose,hemicellulose and lignin reached 7.84%,23.72%and 11.84%,respectively.Further experiments on the methane production potential showed that the biodegradability of vinegar residue was improved effectively by the post-treatment with P.chrysosporium.The optimal methane production was achieved in the experimental group T6-16 with 6 pieces of inoculation amount and 16 days of post-treatment time.The methane production per unit substrate(based on violate solids)of T6-16 reached up to 246.8 mL·g^-1,which was 2.82 times of that in the control group(vinegar residue without post-treatment).This study confirmed the feasibility of post-treatment with P.chrysosporium in promoting the anaerobic digestion efficiency of vinegar residue.
作者
李倩
许之扬
阮文权
LI Qian;XU Zhiyang;RUAN Wenquan(School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China;Jiangsu Key Laboratory of Anaerobic Biotechnology, Wuxi 214122, China)
出处
《浙江农业学报》
CSCD
北大核心
2020年第5期904-911,共8页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(51678279)。
关键词
黄孢原毛平革菌
后处理
醋糟
木质纤维素
厌氧消化
Phanerochaete chrysosporium
post-treatment
vinegar residue
lignocellulose
anaerobic digestion
作者简介
李倩(1995—),女,江苏泰兴人,硕士研究生,研究方向为固体废物资源化,E-mail:1591396811@qq.com;通信作者:阮文权,E-mail:wqruanjn@gmail.com。
