摘要
目的 研究不同种植模式的霍山石斛对小鼠急性肝损伤保护作用.方法 将不同模式种植的新鲜霍山石斛茎条榨汁,以高(生药7.5 g/kg)和低(生药1.25 g/kg)剂量每日灌胃给药,持续2周,使用四氯化碳(CCl4)、对乙酰氨基酚(APAP)和环磷酰胺(CTX)分别诱导急性肝损伤模型.测定小鼠血清中ALT、AST水平,肝组织匀浆中SOD、MDA水平,HE染色检测肝组织病理学变化.结果 3种模式种植的霍山石斛能不同程度减轻小鼠肝细胞坏死.与模型组相比,CCl4肝损伤模型在仿野生和林下石子种植高剂量作用下ALT、AST水平降低(P<0.05),各给药组均能提高SOD水平(P<0.01),抑制MDA水平(P<0.05,P<0.01);APAP肝损伤模型在仿野生种植低、高剂量组和林下石子种植低、高剂量作用下ALT,AST水平降低(P<0.01),林下石子种植低、高剂量组和设施种植高剂量作用下SOD水平升高(P<0.05,P<0.01),MDA水平降低(P<0.01);CTX肝损伤模型在仿野生种植高剂量组和林下石子种植高剂量作用下ALT、AST水平降低(P<0.05,P<0.01),在设施种植高剂量作用下ALT水平降低(P<0.05),在仿野生低、高剂量组和林下石子种植低、高剂量作用下SOD水平升高(P<0.01),在仿野生种植高剂量作用下MDA水平下降(P<0.01).结论 生态种植模式的霍山石斛对小鼠肝损伤的保护作用优于设施种植霍山石斛.
AIM To study the protective effects of differently cultivated Dendrobium huoshanense(DH)on acute liver injury in mice.METHODS High dose(7.5 g/kg)and low dose(1.25 g/kg)squeezed fresh DH juices of different planting methods were administered to mice by oral gavage for 2 weeks.Subsequently the mouse models of acute liver injury were induced by carbon tetrachloride(CCl4),acetaminophen(APAP)and cyclophos phamide(CTX)respectively,and the mice were subjected to the determinations of serum ALT and AST levels,he patic homogenate levels of SOD and MDA,and hepatic pathological changes by HE staining.RESULTS All thedifferently cultivated DH reduced liver cell necrosis in mice to varying degrees.Compared with the model group,in the CCl4 liver injury model,the levels of ALT and AST in the high dose group of wild mimicking planting and on stone under forest planting were reduced(P<0.01),and each administration group could increase SOD levels(P<0.01)and inhibit MDA levels(P<0.05,P<0.01);in APAP liver injury model,ALT and AST levels in low dose and high dose groups of wild mimicking planting and on stone under forest planting were reduced(P<0.01),the SOD levels were increased in the low dose and high dose groups of on stone under forest planting and the high dose group of greenhouse planting(P<0.05,P<0.01),and MDA levels were decreased in the low dose and high dose groups of on stone under forest planting and the high dose group of greenhouse planting(P<0.01);in CTX liver injury model,ALT and AST levels in high dose group of wild mimicking planting and on stone under forest planting were reduced(P<0.05,P<0.01),the ALT levels in the high dose group of greenhouse planting were reduced(P<0.05),the SOD level in low dose and high dose groups of wild mimicking planting and on stone under forest planting were increased(P<0.01),and the MDA level in the high dose wild mimicking planting group were de creased(P<0.01).CONCLUSION DH cultivated in ecological planting is superior to DH cultivated in green house in terms of the liver protection in mice with injury.
作者
李志强
周红秋
欧阳臻
戴军
魏渊
韩邦兴
LI Zhi qiang;ZHOU Hong qiu;OUYANG Zhen;DAI Jun;WEI Yuan;HAN Bang xing(College of Pharmacy,Jiangsu University,Zhenjiang 212013,China;Anhui Provincial Engineering Laboratory for Traditional Chinese Medicine Resource Protection and Sustainable Utilization,Lu’an 237012,China;College of Biotechnology and Pharmaceutical Engineering,West Anhui University,Lu’an 237012,China)
出处
《中成药》
CAS
CSCD
北大核心
2020年第5期1155-1162,共8页
Chinese Traditional Patent Medicine
基金
国家重点研发计划项目(2017YFC1700701)
国家中药材产业技术体系(CARS 21)
安徽省杰出青年科学基金项目(1808085J17)
中央本级重大增减支项目(2060302)。
关键词
霍山石斛
种植模式
生态种植
设施种植
肝损伤保护
Dendrobium huoshanense(DH)
planting pattern
ecological planting
greenhouse planting
liver protection
作者简介
李志强(1995-),男,硕士生,从事中药学研究。Tel:15751000685,E mail:1462493680@qq.com;通信作者:韩邦兴(1978-),男,博士,教授,从事中药资源开发与利用研究。E mail:hanbx1978@sina.com。
