摘要
为了探明不同外源物质处理对干旱胁迫下工业大麻苗期的影响.研究以‘云麻1号’为试验材料,通过盆栽控水法模拟干旱胁迫,研究不同质量浓度(200、500、800 mg/L和1 200 mg/L)的Vc、甜菜碱、CaCl2和赤霉素喷施处理下对工业大麻苗期抗旱的生理效应.结果表明:与对照相比,干旱胁迫处理使‘云麻1号’苗期的叶绿素、脯氨酸和可溶性糖的含量降低,使SOD和POD酶活性也降低.外源物质Vc、甜菜碱、CaCl2和赤霉素处理均可提高大麻苗的叶绿素、脯氨酸和可溶性糖的含量,同时也提高SOD和POD酶活性,从而在一定程度上缓解干旱胁迫对幼苗的伤害.根据隶属函数评价结果得出,‘云麻1号’分别在喷施500 mg/L Vc和CaCl2时生理抗旱特性较好,在喷施200 mg/L甜菜碱和赤霉素时缓解效果也较理想. 4种外源物质缓解效果依次为Vc>CaCl2>赤霉素>甜菜碱.研究结果揭示了干旱胁迫下大麻幼苗对外源物质喷施的生理响应,为外源物质处理提高工业大麻抗旱性的研究提供参考依据.
In order to explore the effects of different exogenous substances on the seedling stage of industrial hemp under drought stress, ‘Yunma 1’ was used as experimental material. Drought stress was simulated by pot water control method. The physiological responses of Vc, betaine, CaCl2 and gibberellin spraying treatments with different concentrations(200, 500, 800 and 1 200 mg/L) to drought resistance of industrial hemp seedlings were studied. The results showed that drought stress reduced the contents of chlorophyll, proline and soluble sugar, and the activities of SOD and POD in ‘Yunma 1’ seedlings compared with the control. Exogenous substances such as Vc, betaine, CaCl2 and gibberellin could increase the contents of chlorophyll, proline and soluble sugar in seedlings, as well as the activities of SOD and POD, thus alleviating the damage of drought stress to seedlings to a certain extent. According to the evaluation result of membership function, ‘Yunma 1’ had better physiological drought resistance when being sprayed 500 mg/L Vc and CaCl2 respectively, and had better mitigation effect being sprayed with 200 mg/L betaine and gibberellin. The alleviating effects of four exogenous substances were Vc >CaCl2 > Gibberellin > Betaine. The results reveal the physiological response of hemp seedlings to exogenous substances spraying under drought stress, and provide a reference for improving drought resistance of industrial hemp by exogenous substance treatment.
作者
杨志晶
李光菊
李璇
黄兴
刘飞虎
杜光辉
YANG Zhi-jing;LI Guang-ju;LI Xuan;HUANG Xing;LIU Fei-hu;DU Guang-hui(School of Agriculture,Yunnan University,Kunming 650500,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第2期374-381,共8页
Journal of Yunnan University(Natural Sciences Edition)
基金
现代农业产业技术体系建设专项(CARS-16-E15)
云南省基础研究计划项目(2015FB108).
关键词
工业大麻
干旱胁迫
苗期
外源物质
生理特性
Cannabis sativa L.
Drought stress
Seedling stage
Exogenous substances
Physiological characteristics
作者简介
杨志晶(1995-),女,云南人,硕士生,主要从事植物组织培养研究;通信作者:杜光辉(1982-),男,山东人,博士,讲师,主要从事工业大麻栽培研究.E-mail:dgh2012@ynu.edu.cn.
