摘要
为研究褪黑素采前喷施番茄植株对采后果实抗灰葡萄孢菌能力和品质的影响,本实验以’Ailsa Craig’番茄为材料,采用1、50、100、150μmol/L褪黑素采前喷施番茄植株,测定采后番茄果实品质、相关酶活力及基因表达量。结果表明:与蒸馏水喷施对照组相比,100μmol/L褪黑素采前喷施处理可以显著降低番茄果实接种灰葡萄孢菌后的发病率、病斑面积(P<0.05),诱导提高果实中几丁质酶、β-1,3-葡聚糖酶、多酚氧化酶和苯丙氨酸解氨酶活力,增加抗病相关基因PR1、NPR1、PIⅡ和LoxD的相对表达量,提高VC、可滴定酸、可溶性糖和可溶性蛋白含量。综上,100μmol/L褪黑素采前处理对采后番茄果实抗灰葡萄孢菌的效果最佳,褪黑素可提高番茄果实的抗病相关酶活性和贮藏品质。
In this study,in order to evaluate the effect of pre-harvest spraying with melatonin(MT)on the resistance to Botrytis cinerea and quality of postharvest fruit,’Ailsa Craig’tomato plants were sprayed with MT at 1,50,100 and 150μmol/L MT before harvest,and then postharvest fruit quality,related enzyme activities and related gene expression levels were determined.The results showed that compared with the control fruit sprayed with distilled water,100μmol/L MT pre-treatment significantly reduced disease incidence and lesion area on the fruit inoculated with Botrytis cinerea(P<0.05),induced increased activities of chitinase,β-1,3-glucanase,polyphenol oxidase and phenylalanine ammonia lyase,increased the expression of the PR1,NPR1,PIⅡand LoxD genes,associated with disease resistance,and elevated the contents of vitamin C,titratable acid,soluble sugar and soluble proteins.In summary,100μmol/L MT pre-treatment had the best effect on enhancing the disease resistance of post-harvest tomato fruit by increasing the related enzyme activities and thus on improving the storage quality.
作者
生吉萍
赵瑞瑞
陈玲玲
申琳
SHENG Jiping;ZHAO Ruirui;CHEN Lingling;SHEN Lin(School of Agricultural Economics and Rural Development,Renmin University of China,Beijing 100872,China;College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2020年第9期188-193,共6页
Food Science
基金
中国人民大学科学研究基金重大项目(18XNL011)。
关键词
番茄果实
褪黑素
采前喷施
采后
抗病性
贮藏品质
tomato fruit
melatonin
pre-harvest spraying
post-harvest
disease resistance
storage quality
作者简介
第一作者:生吉萍(1967-)(ORCID:0000-0003-1259-6350),女,教授,博士,研究方向为食品科学与食品经济管理。E-mail:shengjiping@126.com;通信作者:申琳(1964-)(ORCID:0000-0002-7458-2133),男,副教授,博士,研究方向为果蔬采后生理与生物技术。E-mail:shen5000@cau.edu.cn。
