摘要
目的分析2018年深圳市龙华区诺如病毒基因型特征,为辖区诺如病毒预防和控制策略的制定和调整提供科学依据。方法采用荧光定量RT-PCR方法对哨点医院送检的105份急性胃肠炎临床诊断病例的粪便样本和22起聚集性疫情采集的252份肛拭子样本进行诺如病毒、轮状病毒、星状病毒、肠腺病毒和札如病毒核酸检测,并对诺如病毒核酸阳性样本进行聚合酶-衣壳区序列测定,同源性和系统进化分析。结果哨点监测结果显示,阳性率为21.9%(23/105),单一病原感染中,诺如病毒GⅡ组构成比为47.8%(11/23),GⅠ组构成比为4.4%(1/23),轮状病毒A组构成比为26.1%(6/23),混合感染中,诺如病毒GⅠ和GⅡ组混合构成比17.4%(4/23),诺如病毒GⅡ组和轮状病毒A组混合构成比4.4%(1/23);疫情检测结果显示,由诺如病毒GⅠ组感染引起有1起(4.5%),GⅡ组感染引起的有15起(68.2%),由腺病毒感染有1起(4.5%),未检出的有5起(22.7%);诺如病毒基因特征分析显示,2018年深圳龙华区诺如病毒流行的基因型包括了GⅠ.P1-GⅠ.1,GⅠ.Pd-GⅠ.3,GⅠ.P4-GⅠ.5,GⅡ.P17-GⅡ.17,GⅡ.P16-GⅡ.2,GⅡ.P21-GⅡ.13,GⅡ.P12-GⅡ.4,GⅡ.P12-GⅡ.17和GⅡ.P12-GⅡ.3和GⅡ.P7-GⅡ.6。2018年深圳市龙华区诺如病毒聚集性疾情主要聚集于3月和9-11月。结论诺如病毒是深圳市龙华地区2018年引起急性胃肠炎主要病原,必须持续诺如病毒流行株监测及流行规律研究,为诺如病毒防控策略制定和疫苗株提供数据支持。
Objective To analyze the genotypic characteristics of Norovirus in Longhua District of Shenzhen city in 2018,and provide scientific basis for the formulation and adjustment of Norovirus prevention and control strategies. Methods Stool samples from 105 clinical cases of acute gastroenteritis and 252 samples from 22 cluster cases were collected by fluorescence quantitative RT-PCR. A anal swab sample was tested for Norovirus,Rotavirus,Astrovirus,Enteroadenovirus and Zarovirus nucleic acids,and a Norovirus nucleic acid positive sample was collectedsequencing,homology and phylogenetic analysis of the synthase-capsid region. Results Sentinel surveillance showed a positive rate of 21.9%(23/105). In single-pathogen infection,the composition ratio of GⅡ was 47.8%(11/23). The composition ratio of GⅠ was4.4%(1/23);RV-A was 26.1%(6/23);GⅠ and GⅡ groups mixed composition ratio was 17.4%(4/23),GⅡ and RV-A mixed composition ratio was 4.4%(1/23). The outbreak test showed 1 case of Norovirus(GⅠ)infection(4.5%)and 15 cases of GⅡ infection(68.2%),1 case was caused by Adenovirus infection(4.5%),and 5 cases were not detected(22.7%). Genetic profiling shows that it includes GⅠ.P1-GⅠ.1,GⅠ.Pd-GⅠ.3,GⅠ.P4-GⅠ.5,GⅡ.P17-GⅡ.17,GⅡ.P16-GⅡ.2,GⅡ.P21-GⅡ.13,GⅡ.P12-GⅡ.4,GⅡ.P12-GⅡ.17,GⅡ.P12-GⅡ.3 and GⅡ.P7-GⅡ.6. Shenzhen Longhua district Norovirus clustering epidemic in March and September-November 2018. Conclusion Norovirus was the major pathogen of acute gastroenteritis in Longhua district of Shenzhen city in 2018. The surveillance and prevalence of Norovirus strains should be monitored for the selection of Norovirus vaccine strains.
作者
任燕
许少坚
陈宏标
赖晓玲
黄晓云
满云翔
郝夏琼
李锦宗
刘佳娴
全德甫
曹赫
林启辉
REN Yan;XU Shao-jian;CHEN Hong-biao;LAI Xiao-ling;HUANG Xiao-yun;MAN Yun-xiang;HAO Xia-qiong;LI Jin-zong;LIU Jia-xian;QUAN De-fu;CAO He;LIN Qi-hui(Longhua District Center for Disease Control and Prevention,District Key Laboratory for Infectious Disease Prevention and Control,Shenzhen,Guangdong 518109,China)
出处
《热带医学杂志》
CAS
2020年第2期203-207,共5页
Journal of Tropical Medicine
基金
深圳市三名工程项目(SZSM201809085)。
作者简介
任燕(1980-),女,硕士,副主任技师,从事卫生检验工作,共同第一作者;任燕,共同第一作者;通讯作者:林启辉,E-mail:294769403@qq.com。
