摘要
研究PNU-282987对大鼠脑缺血再灌注损伤的神经保护作用和可能机制。建立体内大脑中动脉闭塞再灌注(MCAO/R)损伤模型,SD大鼠44只随机分为4组:假手术组、模型对照组、PNU-282987低剂量(1.2 mg/kg)和PNU-282987高剂量(2.4 mg/kg)治疗组。Y-型电迷宫检测大鼠空间学习记忆能力,并检测大鼠脑梗死面积、脑含水量和神经功能评分,苏木精-伊红(H&E)染色观察脑组织病理学改变,TUNEL法检测神经元凋亡情况。结果显示,治疗组较模型对照组学习记忆能力明显改善,且治疗组可显著降低缺血再灌注后大鼠脑梗死面积、脑含水量和神经行为学评分,同时发现PNU-282987高剂量(2.4 mg/kg)能够改善缺血脑组织病理学损伤,抑制神经元凋亡。本研究提示PNU-282987可提高脑缺血再灌注损伤后大鼠学习记忆能力,其机制可能与抑制神经元凋亡有关。
To investigate the neuroprotective effect and possible molecular mechanism of PNU-282987 on rats subjected to ischemia and reperfusion.In this study,middle cerebral artery occlusion/reperfusion(MCAO/R)in rats was used as the animal model.The 44 Sprague-Dawley(SD)rats were divided into 4 groups,sham group,model group,low-dose of PNU-282987(1.2 mg/kg)and high-dose of PNU-282987(2.4 mg/kg)treatment group.Y-maze test was tested for the learning and memory abilities of rats,and we also examined the brain infarct size,brain edema and neurological dysfunction in rats.Furthermore,HE staining was used to evaluate the neuronal injury and TUNEL assay was used to evaluate the neuronal apoptosis in the rat brain.The results revealed that the learning and memory abilities of rats in treatment group improved significantly,and treatment with PNU-282987 reduced brain infarct size,lessoned brain edema,lessened neurological dysfunction,ameliorated pathological injury and prevented neuronal apoptosis.The above results suggest that the underlying mechanism of PNU-282987 on improving learning and memory abilities of rats after cerebral ischemia and reperfusion may include the inhibition of neuronal apoptosis.
作者
李晓敏
樊文香
LI Xiaomin;FAN Wenxiang(Department of Pharmacy,Zhongda Hospital,Southeast University,Nanjing 210009,China)
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2020年第2期193-197,共5页
Journal of China Pharmaceutical University
基金
中央高校基本科研业务费专项资金资助(No.2242020K40175)。
作者简介
通讯作者:樊文香,Tel:025-83262638,E-mail:wenxiangfancpu@163.com。
