摘要
为研究山东地区猪繁殖与呼吸综合征病毒(PRRSV)流行病毒株的遗传变异情况,本研究从山东省威海市某疑似猪繁殖与呼吸综合征(PRRS)发病猪场采集的猪肺脏组织中分离到1株PRRSV,命名为SDwh,并对其进行了全基因组序列测定、遗传演化分析和重组分析.结果显示:该病毒株在Marc-145细胞上生长良好,可见明显细胞病变;全基因组演化分析和同源性比对分析结果显示,SDwh株与美国病毒株NADC30和中国的NADC30-like位于同一分支;与北美病毒株NADC30的同源性最高,为93.1%;与我国分离的NADC30-like病毒株CHsx1401、JL580的同源性分别为91.1%和88.4%;与北美经典病毒株VR2332、中国HP-PRRSV JXA1和HuN4的同源性均为85.3%;与欧洲型病毒株Lelystad-virus(LV)同源性最低,为60.6%.与VR2332相比,Nsp2氨基酸序列比对结果显示,SDwh株存在NADC30-like病毒株典型的131个不连续氨基酸的缺失,同时在584~585位缺失2个氨基酸;GP5氨基酸序列比对结果显示,SDwh在GP5抗原表位上有氨基酸的突变.全基因组重组分析结果显示,SDwh株是一株重组病毒株,为NADC30与JXA1-P80的重组病毒,潜在的重组位点位于Nsp2中(2066 nt).本研究结果为PRRSV流行株的重组、演化分析和防控提供借鉴意义.
To understand the genetic variation of Porcine reproductive and respiratory syndrome virus(PRRSV),a PRRSV strain SDwh was isolated in Marc-145 cells from the lung of a pig suspected PRRSV infected in Weihai of Shandong province and its complete genome sequence was amplified by PCR for genetic evolution and recombination analysis.The strain SDwh developed cytopathic effect(CPE)in Marc-145 cells.Phylogenetic analysis showed that the strain SDwh belonged to the same cluster with the NADC30 strain from American and NADC30-like strains from China.In addition,the SDwh had nucleotide homology of 93.1% with the American strain NADC30 and 91.1% and 88.4% with the Chinese NADC30-like strains CHsx1401and JL580.The nucleotide homology of the isolate SDwh was 85.3% with the American classical strain VR2332 and Chinese HP-PRRSV strains JXA1 and HuN4.As expected,the isolate SDwh had the low nucleotide homology of 60.6% with LV strain.The alignment of deduced amino acid sequences revealed that the isolate SDwh had 131 discontinuous amino acid deletion typical for NADC30-like strains and 2 amino acid deletion at 584-585 positions in Nsp2 and amino acid mutations in GP5 as compared with VR2332.These results suggested that the isolate SDwh was a recombinant strain between NADC30 and JXA1-P80 and the potential recombination site was located in Nsp2(2066 nt).This study provided additional information on recombination and evolution of PRRSV in Chinese pig population.
作者
邓紫艳
张锋
董雅琴
吴发兴
段纲
李晓成
DENG Zi-yan;ZHANG Feng;DONG Ya-qin;WU Fa-xing;DUAN Gang;LI Xiao-cheng(China Animal Health and Epidemiology Center,Qingdao 266032,China;College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China)
出处
《中国动物传染病学报》
CAS
北大核心
2020年第3期21-28,共8页
Chinese Journal of Animal Infectious Diseases
基金
科技基础性工作专项(2012FY111000)。
关键词
猪繁殖与呼吸综合征病毒
分离鉴定
重组
演化分析
Porcine reproductive and respiratory syndrome virus
isolation and identification
recombination
genetic variation
作者简介
邓紫艳,女,硕士研究生,预防兽医学专业;张锋,男,博士,助理研究员,主要从事动物疫病流行病学研究;通信作者:李晓成,E-mail:lxch20062006@163.com。
