摘要
为揭示外界环境中营养物质对细菌运动和附着行为特征的影响,以给水管网中常见的铜绿假单胞菌(Pseudomonas aeruginosa)野生株(WT PAO1)和变异株(无运动能力,PAO1ΔfliC)为研究对象,采用基于个体的数学模型方法,使用MATLAB平台模拟微尺度下营养条件胁迫与细菌初期表面附着、个体运动、生长的定量关系,研究其在不同营养环境中的附着与运动行为。结果表明:营养条件显著影响着环境中细菌的运动速度和附着量(P<0.05);贫营养条件下(1/50 LB环境),野生株铜绿假单胞菌的运动速度加快,其表面附着量和附着率提高。在各营养条件下,野生株细菌的附着量和附着率明显高于变异株细菌,验证了运动功能对其表面附着的重要性。通过数学模型建立营养条件与细菌生长繁殖、运动速度的定量关系,揭示给水管网中营养条件调控着细菌和细菌、细菌和表面的碰撞频率以及细菌附着行为。
To explore the effects of nutritional conditions on bacterial motility and surface attachment,Pseudomonas aeruginosa WT PAO1 and PAO1ΔfliC(non-motile mutant)as a model bacterium was used to study cell motility and attachment under various nutrient conditions in drinking water distribution system.To interpret the experimental results,a model was proposed by linking initial surface attachment with cell motility and growth at small scales via individual-based modeling via MATLAB platform.Results showed that nutritional conditions shaped significantly bacterial motility and surface attachment.Nutritional limitation stimulated bacterial motility and thus favored surface attachment for motile cells.In contrast,reducing nutrient concentrations yielded a decline of surface attachment for non-motile cells.Under various nutritional conditions,attached cells and ratios of WT PAO1 cells were significantly higher than those of PAO1ΔfliC,validating the crucial role of cell motility on surface attachment.The quantitative analysis of bacterial growth,motility and interactions with nutritional conditions would be beneficial for predicting nutritional conditions-mediated cell-cell and cell-surface collisions.It will provide mechanistic understanding of bacterial aggregation and attachment process.
作者
杜邦
胡清源
谷正
单蓉蓉
刘丽
DU Bang;HU Qingyuan;GU Zheng;SHAN Rongrong;LIU Li(School of Civil Engineering,Hefei University of Technology,Hefei 230009,China)
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2020年第2期56-62,共7页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(51479046)。
关键词
给水管网
细菌运动
细菌附着和聚集
动力学模拟
生物膜
铜绿假单胞菌
营养条件
drinking water distribution system
bacterial motility
bacterial attachment and aggregation
kinetic simulation
biofilm
Pseudomonas aeruginosa
nutrient condition
作者简介
杜邦,硕士研究生,研究方向:生物膜形成机制与调控,E-mail:dupont33@sina.com;通信作者:刘丽,博士,教授,研究方向:给排水管网安全理论与技术,E-mail:lliu@hfut.edu.cn。
