摘要
探讨定志散对抽动-秽语综合征小鼠脑匀浆中多巴胺含神经递质量和刻板行为的变化.将清洁级ICR小鼠随机分为空白组、模型组、阳性药组、定志散低(1.2g/kg)、中(2.4g/kg)、高(4.8g/kg)剂量组,每组8只.通过腹腔注射3,3’-亚氨基二丙腈建立抽动症模型,在第7、14、21天对各组小鼠进行点头次数测定,21 d进行分类刻板行为评估.21 d断头处死后,采用高效液相-电化学法测定小鼠脑匀浆中含多巴胺的量.小鼠刻板行为结果显示,与空白组相比,模型组刻板行为显著增加(P<0.05);与模型组相比,阳性药组、定志散低剂量组和高剂量组的刻板行为显著降低(P<0.05).小鼠脑匀浆中含多巴胺量显示,与空白组比较,模型组小鼠脑中含多巴胺量显著降低(P<0.05);与模型组比较,高剂量组和阳性药组的小鼠脑中含多巴胺量显著升高(P<0.05).定志散能显著减少抽动-秽语综合征模型鼠的刻板行为,并能改善增加抽动症模型鼠脑中含多巴胺量.
To investigate the effects of Ding Zhi San to the dopamine neurotransmitter and behavioral changes in the brain of mice which has a tourette’s syndrome.Mice were randomly divided into model group,control group,low(1.2 g/kg),medium(2.4 g/kg),high(4.8 g/kg)does Ding Zhi San group and tiapride group.And measure the nodding stereotyped behavior after the model was established by 3,3’-Iminodipropionitrile at 7th day,14th day and 21st day and the dopamine level by HPLC,which was in the brain.The performance of the nodding stereotyped behavior in mice showed that the severity of nodding stereotypes in the model group at 14th day increased significantly(P<0.05)at 21st increased very significantly(P<0.01)as compered to the control group.And the nodding stereotypes in the either tiapride group at 14th day or the high does Ding Zhi San group at 21st day decreased significantly(P<0.05)and in the low does Ding Zhi San group at 21st day decreased very significantly(P<0.01)as compared to the model group.This paper of measured the level of the dopamine in the mice brain showed that the dopamine level in the model group decreased significantly(P<0.05)as compered to the control group.And the dopamine level in both the high does Ding Zhi San group and the tiapride group increased significantly(P<0.05)as compared to TS model group.The nodding stereotypical behavior of mice was linked to the dopamine levels in their brains.Ding Zhi San can significantly reduce the stereotypical behavior of mice with tic model,and increase the level of dopamine in the brain of mice with tic model.
作者
单麒元
刘琳
孙竺
徐静东
SHAN Qi-yuan;LIU Lin;SUN Zhu;XU Jing-dong(School of Pharmacy,Harbin University of Commerce,Harbin 150076,China)
出处
《哈尔滨商业大学学报（自然科学版）》
CAS
2020年第2期142-146,共5页
Journal of Harbin University of Commerce：Natural Sciences Edition
关键词
抽动-秽语综合征
刻板行为
定志散
多巴胺
亚氨基二丙腈
tourette’s syndrome
stereotyped behavior
Ding Zhi San
dopamine
3,3’-iminodipropionitrile
作者简介
单麒元(1997-),男,研究方向:中药药理学;通信作者:刘琳(1978-),女,博士,副教授,研究方向:神经药理学.
