摘要
为探究枳椇子对急性酒精性肝损伤细胞的作用及其机制。首先,SD大鼠枳椇子水提液及生理盐水灌胃后心脏取血,制备枳椇子含药血清及空白血清。然后用酒精诱导人LO_2细胞构建酒精损伤细胞模型,并随机将细胞分为正常组、模型组、空白血清组和不同剂量含药血清组,采用MTT法检测细胞存活率;酶标法检测丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)的酶活力,及丙二醛(MDA)含量;WST-1法检测超氧化物歧化酶(SOD)活性,最后分析评估枳椇子对急性酒精性肝损伤的保护作用。结果显示,与空白血清组比较,5%和10%含药血清组肝细胞存活率显著升高(P<0.05);ALT和AST活性显著降低(P<0.05);MDA含量明显降低,SOD活性显著升高(P<0.05)。表明枳椇子对酒精诱导的肝细胞损伤有一定的保护作用,可能通过抗氧化作用的机制来实现其保护作用。
This study was conducted to investigate the effects of Hovenia dulcis Thunb-containing serum(HDT-containing serum)on anti-oxidation in LO2 cells injured by ethanol and the underlying mechanism.Firstly,total of 16 Sprague-Dawley(SD)rats were randomly divided into control group and drug group.HDT-containing sera were prepared 2 hours after the gastric perfusion at 7th day.Then the LO2 cells used in the experiment were divided into the following groups:normal group,model group,control group,and drug group.Cells were injured in all groups except the normal group.Cell viability was detected using methyl-tetrazolium(MTT)chromatometry,and the degree of cell damage were analyzed measuring the levels of alanine aminotransferase(ALT),aspartate aminotransaminase(AST),malondialdehyde(MDA),and superoxide dismutase(SOD).The results showed that compared with the model group,the 5%and 10%HDT-containing serum groups had significantly higher survival rates(P<0.05),and significantly reduced ALT and AST activity(P<0.05).All serum groups had significantly decreased MDA content and significantly increased SOD activity(P<0.05).The data suggests that pre-treatment with HDT-containing sera has anti-oxidation effect on the LO2 cells injured by ethanol.
作者
黄劲
高霄
雷依丽
胡婷
康颖倩
覃容贵
HUANG Jin;GAO Xiao;LEI Yi-li;HU Ting;KANG Ying-qian;QIN Rong-gui(School of Basic Medical Sciences,Guizhou Medical University,Guiyang 550025,China;Key Laboratory of Environmental Pollution Monitoring and Disease Control of Ministry of Education,Guizhou Medical University,Guiyang 550025,China;School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 550025,China)
出处
《中国兽医杂志》
CAS
北大核心
2019年第11期104-107,共4页
Chinese Journal of Veterinary Medicine
基金
贵州省科技厅中药现代化科技产业研究开发专项项目(黔科合SY字[2014]3008-5)。
关键词
枳椇子
含药血清
LO_2细胞
急性酒精性肝损伤
抗氧化作用
Hovenia dulcis Thunb
Drug-containing serum
LO2 cells
Acute alcohol liver damage
Anti-oxidation effect
作者简介
黄劲(1970-),女,副教授,硕士,研究方向为氧化应激机制,E-mail:huangin@gmc.edu.cn;通讯作者:覃容贵,E-mail:1346812934@qq.com。
