摘要
为揭示田间感染赤星病烟草叶际细菌属水平上的多样性,提取了感病烟叶样品DNA,PCR扩增样品的16S区,采用IonS5XL高通量测序技术对扩增片段测序,并对其细菌群落结构进行分析。结果表明,田间感赤星病烟草叶片叶际细菌群落结构较为典型。在门水平上,优势菌门为变形菌门Proteobacteria(71.59%);属水平上,优势菌为泛菌属Pantoea(31.23%)、假单胞菌属Pseudomonas(22.44%)、甲醇杆菌属Methylobacterium(6.17%)、鞘氨醇单胞菌属Sphingomonas(6.02%)、Aureimonas(2.08%)、Falsirhodobacter(1.35%)。因此,感烟草赤星病叶片叶际优势细菌属为泛菌属和假单胞菌属。
In order to analyze the biodiversity of phyllosphere bacterial genus from tobacco leaves infected by brown spot disease in fields,DNA was extracted from diseased tobacco leaves,16 S region was amplified and sequenced.The amplified fragments were sequenced by IonS5 XL high-throughput sequencing technology,and the bacterial community structure was analyzed.The results showed that the community structure of phyllosphere bacteria from tobacco leaves infected by brown spot disease was quite typical.At the phylum level,the predominant bacteria were Proteobacteria(71.59%);at the genus level,the predominant bacteria included Pantoea(31.23%),Pseudomona(22.44%),Methylobacterium(6.17%),Sphingomonas(6.02%),Aureimonas(2.08%)and Falsirhodobacter(1.35%).All these bacteria were the symbiotic bacteria from tobacco leaves infected with brown spot disease.
作者
刘畅
汪汉成
谢红炼
向立刚
黄宇
陈乾丽
余知和
邹光进
LIU Chang;WANG Hancheng;XIE Honglian;XIANG Ligang;HUANG Yu;CHEN Qianli;YU Zhihe;ZOU Guangjin(College of Life Sciences,Yangtze University,Jingzhou 434025,Hubei,China;Guizhou Academy of Tobacco Science,Guiyang 550081,China;College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China;College of Agriculture,Guizhou University,Guiyang 550081,China;Qiandongnan Branch of Guizhou Provincial Tobacco Company,Kaili 556000,Guizhou,China)
出处
《烟草科技》
EI
CAS
CSCD
北大核心
2020年第2期8-14,共7页
Tobacco Science & Technology
基金
国家自然科学基金项目“基于表型组学的嘧菌酯对烟草赤星病菌作用机理及其抗性机理的研究”(31360448)
贵州省科技支撑计划项目“密集烘烤过程中烟叶腐烂病病害成因及防控技术研究”(黔科合支撑[2018]2356)
贵州省科技厅优秀青年人才培养计划项目“集约化育苗下烟草立枯病的流行规律及其防控技术研究”(黔科合平台人才[2017]5619)
中国烟草总公司贵州省公司科技项目“‘互联网+烟草植保’的研究与应用”(201714)
中国烟草总公司贵州省公司科技项目“贵州烟草叶斑类病害成灾规律与绿色防控技术研究示范”(2019020)。
关键词
烟草赤星病
叶际
细菌
群落结构
高通量测序
Tobacco brown spot disease
Phyllosphere
Bacterium
Community structure
High-throughput sequencing
作者简介
刘畅(1998-),在读硕士研究生,研究方向:微生物学。E-mail:ymliu19@163.com;通信作者:汪汉成,E-mail:xiaobaiyang126@hotmail.com;通信作者:邹光进,E-mail:zouguangjin1@163.com。
