摘要
筛选出具有抑制二肽基肽酶-4(dipeptidyl peptidase-4,DPP-4)活性的植物乳杆菌BLP12,将其代谢物通过有机溶剂萃取、超滤膜、凝胶色谱和高效液相色谱等一系列方法进行分离纯化,利用底物N-甘氨酰脯氨酰-对硝基苯胺盐酸盐与DPP-4在波长405 nm左右强烈的光吸收测定DPP-4活性,选择抑制活性强的部分用液相色谱-质谱联用仪鉴定样品中的物质,质谱共鉴定出61种物质,其中十二烷基硫酸盐是已有报道的DPP-4抑制剂。
In this study,the metabolites of Lactobacillus plantarum BLP12 with inhibitory activity against dipeptidyl peptidase-4(DPP-4)were separated and purified by organic solvent extraction,ultrafiltration membrane,gel chromatography and high performance liquid chromatography.The substrate N-glycyl-prolyl-p-nitroanilide hydrochloride(Gly-Pro-pNA)was applied to determine DPP-4 activity based on the strong absorption at around 405 nm of the degradation product of Gly-PropNA.The fraction with strong inhibitory activity was selected for chemical composition analysis by liquid chromatography tandem mass spectrometry.A total of 61 substances were identified including dodecyl sulfate,which has been reported as a DPP-4 inhibitor.
作者
董杰
刘鹭
甲承立
朱俊玲
吕加平
DONG Jie;LIU Lu;JIA Chengli;ZHU Junling;LU Jiaping(College of Food Science and Engineering,Shanxi Agriculture University,Jinzhong 030800,China;Engineering Research Centre of System-Nutrition,Beijing Institute of Nutrition Resources,Beijing 100069,China;Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2020年第8期116-122,共7页
Food Science
基金
中央级公益性科研院所基本科研业务费专项(S2016JC01)。
关键词
乳酸菌
代谢物
二肽基肽酶-4
抑制活性
lactic acid bacteria
metabolite
dipeptidyl peptidase-4
inhibition activity
作者简介
第一作者:董杰(1994—)(ORCID:0000-0003-0583-1551),女,硕士研究生,研究方向为食品科学。E-mail:18335441280@163.com;通信作者:朱俊玲(1978—)(ORCID:0000-0003-0796-6783),女,副教授,硕士,研究方向为功能化杂粮食品的研发。E-mail:pheobe78@163.com;通信作者:吕加平(1963—)(ORCID:0000-0001-9338-723X),男,研究员,博士,研究方向为乳品加工技术。E-mail:lvjp586@vip.sina.com。
