摘要
目的:探讨Tenuifoliside B(TB)的拟神经生长因子活性。方法:取低分化PC12细胞,给予不同浓度的TB(0、30、60、120μM),作用24h后MTT法测定细胞存活率;取低分化PC12细胞,给予不同浓度的NGF(0、5、10、20、40、80 ng/mL)和TB(0、30、60、120μM)作用48h后观察细胞形态变化;取低分化PC12细胞,MAPK/ERK通路抑制剂PD98059(30μM)、SAPK/JNK通路抑制剂SP600125(30μM)、p38MAPK通路抑制剂SB203580(7.5μM)作用后,NGF(5ng/mL)和TB(120μM)作用48 h后观察细胞形态变化。结果:TB(30、60、120μM)对PC12细胞增殖无显著抑制作用;TB(30、60和120μM)单用,对细胞神经突起伸长无促进作用,但TB(120μM)可协同低浓度的NGF(5ng/mL)显著促进PC12细胞的神经元性分化(P<0.01),其神经突起分化率与NGF(80 ng/mL)相当。加入抑制剂SB203580后,TB协同NGF促进PC12细胞神经元性分化的活性受到抑制。结论:TB协同NGF促进PC12细胞神经元性分化可能与p38MAPK通路的调节有关。
[Objective]To study the neurotrophic effect of Tenuifoliside B(TB).[Method]PC12 cell line was treated with NGF(0,5,10,20,40,80 ng/mL)and TB(0,30,60,120μM)for 48 h to evaluate the neurotrophic effect of TB,respectively.PC12 cell line was pretreated with PD98059(30μM),SP600125(30μM),SB 203580(7.5μM)and subsequently treated with NGF(5ng/mL)and TB(120μM)for 48 h,respectively.Morphologic change of cells was observed under invert microscope.[Result]TB(30,60,120μM)has no significant inhibitory effect on PC12 cell proliferation.TB at a concentration of 30,60,120μMalone did not induce neuritogenesis.However,TB(120μM)can significantly enhance the neuronal differentiation of PC12 cells with low concentration of NGF(5ng/mL)(P<0.01).The neurite differentiation rate is comparable to NGF(80ng/mL).After the inhibitor SB203580 was added,the activity of TB and NGF in promoting neuronal differentiation of PC12 cells was suppressed.[Conclusion]TB in co llaboration with NGF promoting the neuronal differentiation of PC12 cells may be related to the regulation of p38MAPK pathway.
作者
施琦渊
李钦
张玲希
施菁
褚建波
SHI Qiyuan;LI Qin;ZHANG Lingxi;SHI Jing;CHU Jianbo(Hangzhou Medical College,Hangzhou 310053,China)
出处
《浙江医学教育》
2020年第2期38-40,44,共4页
Zhejiang Medical Education
基金
浙江省医药卫生科技计划项目(编号:2017KY311)
“十三五”浙江省中医药(中西医结合)重点学科建设计划(编号:2017-XK-A43)。
作者简介
施琦渊(1981-),男,浙江余杭人,博士研究生,讲师。研究方向:中药质量控制;通信作者:褚建波,高级实验师,cjb0118@126.com。
