摘要
从虎杖根部分离获得8株内生真菌,经液态培养基发酵后进行HPLC检测,以白藜芦醇苷转化率为指标,从中筛选出具有高效转化能力的2株内生真菌G8和G10,其转化率分别达到64.2%和58.1%,将虎杖中的白藜芦醇含量提高到原来的3.84倍和3.57倍。结合ITS测序与Genback中rDNA同源序列对比,鉴定G8和G10分别为青霉菌RF3(Penicillium sp.RF3)和青霉菌ST008(Penicillium sp.isolate ST008)。
Eight endophytic fungi were isolated from the roots of Polygonum cuspidatum.After fermentation in liquid medium,HPLC was used to determine the resveratrol glycoside conversion rate.Two endophytic fungi G8 and G10 with high-efficienct transformation ability were screened out.The transformation rate of G8 and G10 were reached 64.2% and 58.1%,respectively.In addition,the resveratrol contents of Polygonum cuspidatum were increased to 3.84 times and 3.57 times of the original.Combining ITS sequencing with rDNA homologous sequences in Genback,G8 and G10 were identified as Penicillium sp.RF3 and Penicillium sp.isolate ST008.
作者
邓欣蓓
庞立
蒋立文
李跑
王仁才
张强
易有金
夏菠
DENG Xinbei;PANG L i;JIANG Liwen;LI Pao;WANG Rencai;ZHANG Qiang;YI Youjin;XIA Bo(College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China;College of Horticulture and Landscape,Hunan Agricultural University,Changsha 410128,China;Shouguang Vegetable Industry Group Co.,Ltd.,Shouguang 262700,China)
出处
《食品科技》
CAS
北大核心
2020年第2期11-17,共7页
Food Science and Technology
基金
湖南省自然科学研究基金项目(2018JJ2169)
湖南省自然科学基金项目(2018JJ3231)。
关键词
虎杖
内生真菌
白藜芦醇苷
分离鉴定
Polygonum cuspidatum
endophytic fungus
resveratrol glycoside
isolation and identification
作者简介
邓欣蓓(1995-),女,硕士研究生,研究方向为食品微生物;通信作者:易有金;通信作者:夏菠。
