摘要
目的了解洛阳市外环境中禽流感病毒存在状况、职业暴露人群血清中禽流感病毒抗体水平,为洛阳市人感染禽流感疫情防控、预测和预警提供依据。方法在洛阳市禽流感监测点采集外环境标本,用PCR方法对标本进行禽流感病毒核酸检测;采集职业暴露人群的血清标本,采用血凝抑制实验进行H5N1、H7N9、H9N2抗体检测。结果2012年01月至2018年12月,共采集外环境标本587份,检出A型禽流感病毒56份,检出A型禽流感病毒阳性率为9.54%。其中H9亚型阳性率7.50%,H5亚型阳性率0.00%,H7亚型阳性率0.00%,其他A型(排除H5、H7、H9)亚型阳性率2.04%。不同类型监测场所,活禽市场及家禽屠宰售卖点禽流感病毒检出率较高;不同标本类型,笼具、案板表面擦拭标本及清洗禽类污水的禽流感病毒阳性率较高;月份分布主要集中在每年的1月~3月及12月,2月高于其他月份。共采集595份职业暴露人群血清进行检测,没有检出H5N1、H7N9、H9N2抗体。结论洛阳市禽类相关外环境中存在禽流感病毒,以H9亚型为主,活禽市场和家禽屠宰售卖点禽流感病毒存在较多;职业暴露人群血清中H5N1;H7N9;H9N2抗体较低。
Objective To understand the status of avian influenza virus in the external environment of Luoyang and the level of avian influenza virus antibody in the serum of occupationally exposed population,so as to provide basis for prevention,control,prediction and early warning of avian influenza in Luoyang City.Methods The samples of external environment were collected at the surveillance site of avian influenza in Luoyang,and the nucleic acid of avian influenza virus was detected by PCR.The serum samples of occupationally exposed population were collected and the antibodies of H5N1,H7N9 and H9N2 were detected by hemagglutination inhibition(HI)test.Results From January 2012 to December 2018,587 samples of external environment were collected and 56 samples of avian influenza A virus were detected.The positive rate of avian influenza A virus was 9.54%.The positive rate of H9 subtype was 7.50%,H5 subtype was 0.00%,H7 subtype was 0.00%,and other subtypes A(excluding H5,H7,H9)were 2.04%.The detection rate of avian influenza virus was higher in live poultry markets and poultry slaughter and sale points in different monitoring sites;the positive rate of avian influenza virus was higher in cages,wiping samples on the surface of case boards and cleaning poultry wastewater in different sample types;the distribution of avian influenza virus was mainly concentrated in January-March and December of each year,and was higher in February than in other months.A total of 595 sera from occupational exposed persons were collected for detection.H5N1,H7N9 and H9N2 antibodies were not detected.Conclusions There are avian influenza viruses in the related external environment of poultry in Luoyang City,mainly H9 subtype.There are more avian influenza viruses in live poultry market and poultry slaughter and sale points.The serum H5N1,H7N9 and H9N2 antibodies of occupational exposure population are low.
作者
张广武
刘笑洁
李莹
孙向南
ZHANG Guangwu;LIU Xiaojie;LI Ying;SUN Xiangnan(Luoyang Center for Disease Control and Prevention,Luoyang,Henan 471000,China)
出处
《河南预防医学杂志》
2020年第4期257-259,共3页
Henan Journal of Preventive Medicine
作者简介
通讯作者:张广武,主管技师,硕士,研究方向:微生物检验,E-mail:439264743@qq.com。
