摘要
目的改进岩果止咳液的质量标准。方法采用薄层色谱(TLC)法对果上叶进行定性鉴别;采用高效液相色谱-紫外检测(HPLC-UV)法同时测定石吊兰、甘草流浸膏中石吊兰素、甘草酸铵的含量。结果TLC斑点清晰,专属性强;石吊兰素、甘草酸铵质量浓度分别在0.004866~0.2433 mg/mL(r=0.9999)和0.0228~1.140 mg/mL(r=0.9997)范围内与峰面积线性关系良好,平均加样回收率分别为98.46%和98.91%,RSD分别为2.25%和0.98%(n=9)。结论该方法灵敏、准确,可完善岩果止咳液的质量标准。
Objective To improve the quality standard of Yanguo Zhike Liquid.Methods Thin layer chromatography(TLC)method was used to identify the denseflower bulbophyllum herb.High performance liquid chromatography-ultraviolet detection(HPLC-UV)method was used to simultaneously determine the content of lysionotin in Lysionotus Pauciflorus and ammonium glycyrrhizinate in Extractum Glycyrrhizae Liquidum.Results TLC spots were clear with strong specificity.The mass concentrations of lysionotin and ammonium glycyrrhizinate were linearly related to the peak area within the ranged of 0.004866-0.2433 mg/mL(r=0.9999)and 0.0228-1.140 mg/mL(r=0.9997),and the average sample recoveries were 98.46%and 98.91%,RSDs were 2.25%and 0.98%respectively(n=9).Conclusion This method is sensitive and accurate,which can improve the quality standard of Yanguo Zhike Liquid.
作者
吕情花
LYU Qinghua(Loudi Food and Drug Inspection and Testing Institute,Loudi,Hunan,China 417000)
出处
《中国药业》
CAS
2020年第7期116-118,共3页
China Pharmaceuticals
关键词
岩果止咳液
果上叶
石吊兰素
甘草酸铵
高效液相色谱-紫外色谱
Yanguo Zhike Liquid
denseflower bulbophyllum herb
lysionotin
ammonium glycyrrhizinate
high performance liquid chromatography-ultraviolet detection
作者简介
第一作者:吕情花,女,大学本科,副主任药师,研究方向为药品检验及中药制剂质量标准,(电子信箱)374556425@qq.com。
