摘要
The signature events caused by host-guest interactions in the nanopore system can be used as a novel and characteristic signal in quantitative detection and analysis of various molecules.However,the effect of several electrochemical factors on the host-guest interactions in nanopore still remains unknown.Here,we systematically studied host-guest interactions,especially oscillation of DNA-azide adamantane@cucurbit[6]inα-Hemolysin nanopore under varying pH,concentration of electrolytes and counterions(Li+,Na+,K+).Our results indicate correlations between the change of pH and the duration of the oscillation signal.In addition,the asymmetric electrolyte concentration and the charge of the counterions affects the frequency of signature events in oscillation signals,and even the integrity of the protein nanopore.This study provides insight into the design of a future biosensing platform based on signature oscillation signals of the host-guest interaction within a nanopore.
基金
the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under award number K22AI136686
the South Carolina IDeA Networks of Biomedical Research Excellence Developmental Research Project funded by the National Institute of GeneralMedical Sciences of the National Institutes of Health.
作者简介
Corresponding author Chang Liu at:Biomedical Engineering Program,Department of Chemical Engineering,University of South Carolina,Columbia,SC 29208,USA.is an Assistant Professor of Biomedical Engineering and Chemical Engineering at the University of South Carolina.Prior to this appointment,he served as an Assistant Research Scientist at the Biodesign Institute of Arizona State University.Dr.Liu completed his Postdoctoral Fellowship in the Department of Nanomedicine at the Research Institute of Houston Methodist Hospital.He received his Ph.D.(2013)in Biomedical Engineering from Florida International University.Dr.Liu's research focuses on applying nanotechnology on the development of biosensors for disease diagnosis.E-mail address:changliu@cec.sc.edu(C.Liu);XiaojunWei received his Ph.D.(2018)in Physical Chemistry at the Institute of Chemistry,Chinese Academy of Sciences.He is currently working as a Postdoctoral Fellow at the College of Engineering and Computing,University of South Carolina.His research focus is the application of nanopore for biomarker detection.
