摘要
为研究曝气生态滤池滤料表面物种多样性及其净水机理,通过高通量测序和生物信息分析对曝气生态滤池(EAF)中2种填料的不同DO区域微生物丰度和多样性进行研究和分析.结果表明,在EAF运行过程中,变形菌门所占比例均在71%以上,具有绝对优势,优势菌群的大量增殖使装置具有较高的TN去除率(67%),其中好氧区海绵铁表面样本优势菌群比例最高,达到77.49%,表明海绵铁在曝气条件下更适合优势菌群变形菌门的繁殖;Alpha多样性分析发现EAF内填料表面生物膜中微生物菌落丰度均高于空白样,表明EAF较自然环境更适合微生物生长;Beta多样性分析发现装置由于曝气使优势菌群变形菌门在填料表面大量繁殖,限制了其他菌种繁殖,虽然生物多样性略有降低,但随着优势菌群大量增殖从而提升了对TN的去除能力.
In order to study the species diversity on the surface of filter material in an ecological aerated filter(EAF)and elucidate its water purification mechanism,microbial abundance and diversity in different dissolved oxygen(DO)regions of two fillers in the EAF were analyzed using high-throughput sequencing and bioinformatics analysis.Proteobacteria was the predominant specie which accounted for more than 71% during the EAF operation.The large proliferation of dominant bacteria enabled the reactorexhibited a high TN removal rate(67%),The ratio of dominant bacteria on the surface samples of sponge iron in aerobic area was the highest(77.49%),indicating that sponge iron was more suitable for the propagation of proteobacteria of dominant bacteria under aeration conditions.Alpha diversity analysis found that the abundance of microbial colonies in the biofilmof the filler surface of EAF was higher than that of the control group,indicating that EAF was more suitable for the growth of microorganisms than the natural environment.The diversity analysis of Beta showed that the aeration in the reactor caused the dominant bacteria to propagate effectively on the filler surface,which suppressed the growth of other bacteria.Although the biodiversity declined slightly,the removal capacity of total nitrogen(TN)was improved with the proliferation of the dominant bacteria.
作者
刘亚妮
朱宏伟
黄荣新
郭志鹏
魏东洋
周雯
LIU Ya-ni;ZHU Hong-wei;HUANG Rong-xin;GUO Zhi-peng;WEI Dong-yang;ZHOU Wen(School of Petrochemical Engineering,Lanzhou University of Technology,Lanzhou 730050,China;State Environmental Protection Key Laboratory of Urban Ecological Environment Simulation and Protection,South China Institute of Environmental Sciences,Ministry of Ecology and Environmental,Guangzhou 510535,China;Zheng Ye Design CO.LTD,Harbin 150000,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2020年第3期1075-1080,共6页
China Environmental Science
基金
国家重点研发计划专项(2016YFC0400703-3)。
关键词
铝污泥陶粒
海绵铁
曝气生态滤池
高通量测序
生物信息分析
aluminum sludge ceramsite
sponge iron
ecological aerated filter
high throughput sequencing
bioinformatics analysis
作者简介
刘亚妮(1994-),女,甘肃平凉人,兰州理工大学硕士研究生,主要从事水污染控制技术研究;责任作者:周雯,高级工程师,zhouwen@scies.org。
