摘要
建立了同时检测大米中6种硒形态的超声辅助酶法提取-高效液相色谱-原子荧光光谱联用检测方法(HPLC-AFS)。利用链霉蛋白酶E,超声提取30min,使用Hamilton PRP-X100色谱柱(250mm×4.1mm,10μm),99%的5 mmol/L pH为4.70柠檬酸的缓冲溶液和1%的甲醇洗脱,原子荧光检测,结果显示:各目标物方法检出限在0.5~3.1μg/L,相关系数≥0.9992,回收率为80%~114%。本方法适用于大米中6种硒形态检测。
Samples were extracted with pronase E by ultrasound for 30 minutes.The separation was performed on a Hamilton PRP-X100 chromatographic column(250mm×4.1mm,10um),1%methanol,99%ammonium acetate(pH=4.70,5 mmol/L)were used as mobile phase.Tested by AFS,the samples were quantitatively determined by external standard method.The measurement results showed that the detection limit was 0.5-3.1μg/L,the correlation coefficient was above 0.9992,and the average recovery rates of methyl mercury and other mercury species across three spike levels were 80%-114%.This method can meet the requirements of 6 kinds of selenium species determination in rice.
作者
曾云想
梁婷婷
汤明河
金璇
俞兴伟
刘国强
Zeng Yunxiang;Liang Tingting;Tang Minghe;Jin Xuan;Yu Xingwei;Liu Guoqing(Leisure Food Quality Inspection Center of Zhejiang,Wenzhou 325000,China;Cangnan Center for Disease Control and Prevention,Wenzhou 325000,China)
出处
《分析仪器》
CAS
2020年第1期20-24,共5页
Analytical Instrumentation
基金
浙江省温州市科技计划项目(G20170036)。
关键词
硒形态
大米
液相色谱-原子荧光光谱
Selenium species
Rice
High performance liquid chromatography-atomic fluorescence spectrometry(HPLC-AFS)
作者简介
曾云想,男,1987年出生,硕士研究生,工程师,主要从事食品理化检验检测,E-mail:zyx0611401136@163.com。
