摘要
目的探讨应用经诱导分化成骨的人羊膜间充质细胞(human amniotic mesenchymal cells,hAMCs)与明胶海绵载体复合培养后进行移植修复兔牙槽突裂的实验效果。方法采用手术方法建立兔牙槽突裂的动物模型,根据移植物内容的不同将建立的牙槽突裂兔随机分为A组(移植hAMCs与胶原-明胶海绵复合体)、B组(移植经诱导分化成骨后hAMCs与胶原-明胶海绵复合体)、C组(移植未作处理的胶原-明胶海绵)、D组(空白对照)。于移植后特定时间段分别处死各实验组动物后,对移植区牙槽骨的厚度进行测量,采用苏木精-伊红染色及免疫组织化学染色观察结果。结果第12周处死的大体标本示A/B两组牙槽突缺损处大量骨质沉积,C/D组缺损处仅少量骨质充填,但D组牙根暴露;第12周后A组与B组移植区牙槽骨厚度比较无统计学差异(P>0.05),A/B组分别与C/D组比较有统计学差异(P<0.01),C组与D组比较有统计学差异(P<0.05);苏木精-伊红染色示A/B两组在术后2周即可见成骨细胞聚集区,而C组各个时期均未发现成骨细胞聚集区;12周后移植区A/B组免疫组化检测到抗人细胞核抗体的细胞存在,C组未检测到抗人细胞核抗体的细胞。结论hAMCs及成骨诱导后的hAMCs均可在体内成活,具有成为组织工程修复牙槽突裂种子细胞的潜力,成骨诱导后的hAMCs与胶原-明胶海绵复合体有望成为牙槽突植骨修复的备选方法之一。
Objective To explore the experimental effect of using human amniotic mesenchymal cells(hAMCs)derived from bone induced differentiation combined with gelatin sponge carrier for transplantation and repair of alveolar cleft in rabbits.Methods The animal model of alveolar cleft in rabbits was established by surgical method.The rabbits were randomly divided into Group A(transplanted hAMCs and Collagen-gelatin sponge complex),group B(transplanted hAMCs and Collagen-gelatin sponge complex after osteogenesis),group C(transplanted untreated Collagen-gelatin sponge),and group D(blank control)according to the different contents of the graft.After killing animals in all experimental groups at a specific time after transplantation,the thickness of alveolar bone staining in the transplantation zone was measured.Hematoxylin-eosin staining and immunohistochemical staining were used to observe the results.Results The gross specimens executed at week 12 showed A large amount of bone deposition at the alveolar defect in group A/B,and only A small amount of bone filling at the defect in group C/D,but the root was exposed in group D.After 12 week,there were no statistical significantly differences in alveolar bone thickness between group A and group B(P>0.05),while there were statistical significantly differences between group A/B and group C/D(P<0.01),and there were statistical significantly differences between group C and group D(P<0.05).Hematoxygen-eosin staining showed that osteoblasts were observed in both groups 2 weeks after the operation,while no osteoblasts were observed in group C at any time.After 12 weeks,the immunohistochemistry of group A/B in the transplantation area detected the presence of cells with anti-human nuclear antibody,while no cells with anti-human nuclear antibody were detected in group C.Conclusion Both hAMCs and hAMCs induced by osteogenesis can survive in vivo,and have the potential to become seed cells of tissue engineering for alveolar cleft repair.HAMCs and Collagen-gelatin sponge complex induced by osteogenesis is expected to be one of the alternative Methods for alveolar process bone grafting repair.
作者
王芳
宋庆高
郭佳男
何苇
陈尚
邹亚莉
Wang Fang;Song Qinggao;Guo Jianan;He Wei;Chen Shang;Zou Yali(Department of Oral and Maxillofacial Surgery,Hospital of Stomatology,Zunyi Medical University,Zunyi 563000,Guizhou,China.)
出处
《贵州医药》
CAS
2020年第2期175-177,I0001,共4页
Guizhou Medical Journal
基金
贵州省科学技术基金(黔科合J字LKZ[2013]29号)。
关键词
人羊膜间充质细胞
牙槽突裂
修复
成骨分化
Human amniotic mesenchymal cells
Alveolar cleft
Repair
Osteogenesis differentiation
作者简介
通信作者:宋庆高,E-mail:E-mail:814641639@qq.com。
