摘要
目的分析2017-2018年成都地区诺如病毒聚集性疫情中病毒基因型构成情况,为疾病防控工作提供依据。方法选取2017-2018年诺如病毒聚集性疫情标本,对病毒基因RdRp及VP1区片段测序,构建进化树并进行同源性分析。结果测序结果显示,68份肛拭子标本中16.2%(11/68)为GⅠ群(包括GⅠ.2、GⅠ.3和GⅠ.5型),83.8%(57/68)为GⅡ群(包括GⅡ.P16-GⅡ.2、GⅡ.P17-GⅡ.17、GⅡ.P8-GⅡ.8、GⅡ.P12-GⅡ.3、GⅡ.P7-GⅡ.6和GⅡ.P15-GⅡ.15型)。2017年以GⅡ.P16-GⅡ.2型为主;2018年GⅠ群及GⅡ.P17-GⅡ.17型均显著增加,同时检出其他4种基因型。各基因型病毒变异不明显,核苷酸同源性为93.4%~100%。结论2018年成都地区诺如病毒流行株基因型构成较2017年复杂,可能与聚集性疫情增长有关。应持续监测诺如病毒基因型流行情况,及时掌握病毒变异动态,以期提高疾病防控的预警能力。
Objective To analyze the composition of viral genotypes in aggregation epidemics induced by norovirus infection in Chengdu region in 2017-2018,and to provide a basis for its prevention and control.Methods Anal swab samples from aggregation epidemics induced by norovirus infection in Chengdu region during 2017-2018 were selected,and then the sequence information of RdRp and VP1 was analyzed.The phylogenetic tree was constructed to analyze the nucleotide sequence homology.Results The sequence analysis revealed that 16.2%(11/68)samples belonged to NoV genogroup GⅠ(including GⅠ.2,GⅠ.3 and GⅠ.5).83.8%(57/68)samples belonged to NoV genogroup GⅡ(including GⅡ.P16-GⅡ.2,GⅡ.P17-GⅡ.17,GⅡ.P8-GⅡ.8,GⅡ.P12-GⅡ.3,GⅡ.P7-GⅡ.6 and GⅡ.P15-GⅡ.15).GⅡ.P16-GⅡ.2 was the main genotype circulating in 2017.Genogroups GⅠand GⅡ.P17-GⅡ.17 significantly prevailed in 2018;meanwhile,other four genotypes were detected.The variation of noroviruses with different genotypes was not obvious.The nucleotide sequence homology ranged from 93.4%to 100%.Conclusions The genotype composition of norovirus strains predominated in Chengdu region in 2018 was more complicated than that in 2017,which might be related to the increase of aggregation epidemics.It is necessary to continuously monitor the molecular epidemiology of norovirus strains and master the variation of norovirus in time so as to improve the early warning ability in disease prevention and control.
作者
程悦
孟建彤
陈恒
付芮
黄薇
梁娴
CHENG Yue;MENG Jian-tong;CHEN Heng;FU Rui;HUANG Wei;LIANG Xian(Chengdu Municipal Center for Disease Control and Prevention,Chengdu,Sichuan 610041,China;Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan 610075,China)
出处
《实用预防医学》
CAS
2020年第3期311-314,共4页
Practical Preventive Medicine
基金
四川省卫生计生委重点项目(17ZD046)
成都市卫生和计划生育委员会项目(2017015)。
作者简介
程悦(1990-),女,四川人,硕士,检验师,主要从事微生物检验及相关工作;共同第一作者:孟建彤;通信作者:梁娴,E-mail:75539392@qq.com。
