摘要
通过十八烷基胺的氨基与二醛微晶纤维素的醛基共价键合,制备了基于二醛微晶纤维素(DMCC)官能化C18的新型反相/亲水色谱固定相(C18-DMCC/SiO2),该色谱固定相被用于反相色谱(RPLC)和亲水相互作用色谱(HILIC)模式。C18-DMCC/SiO2色谱柱展现了良好的疏水选择性和芳香选择性,在反相色谱模式下可分离烷基苯和多环芳烃(PAHs)。苯胺类、酚类和糖苷类等极性化合物被用于评估该色谱柱在反相色谱模式下的极性选择性,商品C18柱作对照柱,色谱评价结果令人满意。核酸碱基被用于评估C18-DMCC/SiO2色谱柱的亲水色谱性能。通过考察有机溶剂含量对分析物保留的影响,发现该新型色谱固定相具有反相/亲水色谱的典型特征。
A novel reversed/hydrophilic chromatographic stationary phase based on dialdehyde microcrystalline cellulose(DMCC)-functionalized C18 was prepared by covalent bonding between the amino groups of octadecylamine with the aldehyde groups of DMCC,which was used in reversed-phase liquid chromatography(RPLC)and hydrophilic interaction liquid chromatography(HILIC)modes. The prepared DMCC-functionalized C18 modified silica(C18-DMCC/SiO2)stationary phase exhibited good hydrophobic selectivity and aromatic selectivity by separating alkylbenzenes and polycyclic aromatic hydrocarbons(PAHs)in the RPLC mode. Polar compounds,including anilines,phenols,and glycosides were chosen as analytes to evaluate the polar selectivity of this column in the RPLC mode,and the evaluation results were satisfactory compared with the commercial C18 column. Nucleobases were used for evaluating the hydrophilic interaction liquid chromatography performance of the C18-DMCC/SiO2 column. By investigating the impact of organic solvent content on the retention,it could be found that this new stationary phase had the typical characteristics of reversed-phase/hydrophilic chromatography.
作者
高洁
武琪
陈立骁
李辉
董树清
罗国英
张书胜
邱洪灯
赵亮
GAO Jie;WU Qi;CHEN Lixiao;LI Hui;DONG Shuqing;LUO Guoying;ZHANG Shusheng;QIU Hongdeng;ZHAO Liang(Key Laboratory of Chemistry of Northwestern Plant Resources,Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China;University of Chinese Academy of Sciences,Beijing 100049,China;College of Chemistry and Molecular Engineering,Zhengzhou University,Zhengzhou 450001,China)
出处
《色谱》
CAS
CSCD
北大核心
2020年第4期414-423,共10页
Chinese Journal of Chromatography
基金
National Natural Science Foundation of China(Nos.21702210,21675163)
关键词
反相色谱
亲水相互作用色谱
高效液相色谱
固定相
制备
二醛微晶纤维素
C18
reversed-phase liquid chromatography(RPLC)
hydrophilic interaction liquid chromatography(HILIC)
high performance liquid chromatography(HPLC)
stationary phase
preparation
dialdehyde micro-crystalline cellulose(DMCC)
C18
作者简介
Corresponding author:QIU Hongdeng,Tel:+86⁃931⁃4968877,E⁃mail:hdqiu@licp.cas.cn;Corresponding author:ZHAO Liang,Tel:+86⁃931⁃4968261,E⁃mail:zhaol@licp.cas.cn。
