摘要
植物MADS盒基因蛋白是一类在生物发育过程中起着重要作用的转录因子家族。本研究在前期大豆花芽转录组测序分析的基础上,利用RACE技术成功克隆了一个MADS-box基因的全长cDNA序列,利用生物信息学方法对它所编码的氨基酸基本性质和结构进行了系统分析。结果表明,该基因属于MIKC家族,为亲水性蛋白,稳定性较好;二级结构中同时含有α-螺旋、β-转角、延伸链和无规则卷曲结构,其中α-螺旋和无规则卷曲所占比例均较高;亚细胞定位分析显示其定位在细胞核上;聚类分析表明该基因属于AP3亚家族,该基因编码的氨基酸与拟南芥AP3蛋白的同源性为41.18%,与大豆中GmNMH7的同源性最高为97%,命名该基因为GmAP3。本研究成功克隆出GmAP3基因,为该基因的进一步研究提供实验材料,并通过生物信息学分析预测,为下一步该基因的功能研究提供依据。
Plant MADS box gene proteins are a family of transcription factors that play an important role in biological development.On the basis of transcriptome sequencing analysis of soybean flower bud in the early stage,a full-length cDNA sequence of MADS-box gene was cloned using RACE technology.In order to reveal the function of this gene,the property and structure of proteins were analyzed using bioinformatics methods.The results showed that this gene belongs to the MIKC family.It was a stable hydrophilic protein.The secondary structure contains alpha helix,beta turn,extended strand and random coil,among of them alpha helix and random coil were major part.The subcellular localization analysis showed that it was located in the nucleus.Cluster analysis showed that this gene belongs to the AP3 subfamily.The amino acid homology of the gene was 41.18%with AP3 protein of Arabidopsis thaliana,and the highest homology was 97%with GmNMH7 in soybean.The gene was named GmAP3.The GmAP3 gene was successfully cloned to pro vide experimental materials for further research of this gene,and it was predicted by bioinformatics analysis to provide a basis for the next functional study of the gene.
作者
杨桐珲
刘占柱
管翊君
焦苏琪
李广隆
姚丹
Yang Tonghui;Liu Zhanzhu;Guan Yijun;Jiao Suqi;Li Guanglong;Yao Dan(College of Life Sciences,Jilin Agricultural University,Changchun,130118)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第3期729-736,共8页
Molecular Plant Breeding
基金
吉林省科技发展计划项目(20180101266JC)
吉林省教育厅“十三五”科学技术项目(JJKH20180641KJ)
农业农村部东北作物基因资源与种质创制重点实验室——开放课题(CXGC2018KFKT006-3)共同资助。
作者简介
通信作者:姚丹,yaodan@jlau.edu.cn。
