摘要
以自组装方法,构建了Au纳米粒子(AuNPs)/寡核苷酸/硅量子点(SiQDs)复合物荧光传感体系,AuNPs使复合物中咪唑基硅量子点荧光猝灭。样品溶液中存在黄曲霉毒素B1(AF B1)时,复合物中寡核苷酸与AF B1发生特异性反应,释放出咪唑基硅量子点,使体系的荧光得到恢复,并且其荧光强度随加入样品中AF B1量的增大而增强。优化实验条件为缓冲溶液pH=7.5,50 mmol/L NaCl,孵化时间5 min。在最优条件下,AF B1浓度在0.01~1.0 ng/mL范围与体系荧光强度恢复程度呈现出良好的线性关系,方法检测限(3σ)为8 pg/mL。该方法已成功应用于实际样品中AF B1的测定,其回收率在94.0%~106.0%范围,相对标准偏差为2.1%~3.5%。
The nanocomposites of Au nanoparticles/oligonucleotides/silicon quantum dots were prepared by self-assembly.Fluorescence of silicon quantum dots with imidazole groups was quenched by Au nanoparticles in the nanocomposites.Then the fluorescence of the system was recovered when aflatoxin B1(AF B1)was added to the nano-composites solution as the specific reactions between AF B1 and the oligonucleotide in the nanocoposites caused the release of silicon quantum dots with imidazole groups.The fluorescence of the system increased with the increase of AF B1 concentration.Based on the above facts,a sensitive fluorescence biosensor for determination of AF B1 was developed,and the optimal conditions for AF B1 detection were as follows:buffer solution pH of 7.5,50 mmol/L NaCl and incubation for 5 min.Under the optimal conditions,the fluorescence intensity signal change(F-F 0)and the AF B1 concentration showed a linear correlation within 0.01 to 1.0 ng/mL range with a detection limit of 8 pg/mL(3σ).This method was successfully employed to detect AF B1 in chocolate samples with recoveries of 94.0%-106.0%,and the relative standard deviation was of 2.1%-3.5%.
作者
易守军
黎燕
张敏
何盼
赵敏
唐春然
曾云龙
YI Shoujun;LI Yan;ZHANG Min;HE Pan;ZHAO Min;TANG Chunran;ZENG Yunlong(Key Laboratory of Theoretical Organic Chemistry and Functional Molecule,Ministry of Education,Hunan Province Key Laboratory of Controllable Preparation and Function Application of Fine Polymer,School of Chemistry and Chemical Engineering of Hunan University of Science and Technology,Xiangtan 411201;School of Materials Science and Engineering of Hunan University of Science and Technology,Xiangtan 411201)
出处
《分析科学学报》
CAS
CSCD
北大核心
2020年第1期47-51,共5页
Journal of Analytical Science
基金
国家自然科学基金(No.21475040)
关键词
硅量子点
寡核苷酸
荧光传感
黄曲霉毒素B1
Silicon quantum dots
Oligonucleotides
Fluorescence sensors
Aflatoxin B1
作者简介
通讯作者:曾云龙,男,博士,教授,研究方向:量子点荧光材料合成、化学生物传感。E-mail:yunlongzeng1955@126.com。
