摘要
[目的]找到苏木精和伊红以外其它染色效果良好的组织切片染色剂。[方法]应用27种直接服装染料对小鼠肝脏组织切片进行染色效果观察。发现其中的活性玫红染料染色能够清晰显示中央静脉和肝细胞核等结构。再用活性玫红染料对小鼠心脏、肾脏、睾丸和肺组织切片进行染色效果观察,同时用伊红进行染色对比观察。[结果]活性玫红染料用蒸馏水溶解后即可使用,配制简单,在5 min之内完成。对心脏、肾脏、睾丸和肺组织切片染色后,肾小囊腔、生精细胞等结构清晰可辨,与HE染色结果类似。心肌横纹也可辨认。活性玫红处理的肝脏、肾脏和睾丸组织切片染色清晰度,高于伊红的染色效果。[结论]找到活性玫红染料这种染色效果良好的红色组织切片染色剂,其制备简单,来源丰富。
[Objective]To find out other effective and abundant tissue slice dye apart from hematoxylin and eosin.[Method]27 kinds of directly used dress dye were tested to make mouse liver slice stained.Among them Active Rose red made hepatic structure including central vein and hepatic nucleus shown clearly.Then,the heart,kidney,testis and lung slices of mouse were stained by Active Rose red and eosin as control.[Result]The solution preparation of Active Rose red is just dissolved in distilled water in five minutes,so it is very much simple and time-saving.After the heart,kidney,testis and lung slices of mouse were stained by Active Rose red,the stained tissue structure including cardiac muscle cross striation,Kidney capsule cavity and spermatogenic cells is clear and similar to tissue stained by HE.The resolution of the liver,kidney and testis slices stained by Active Rose red is much better than by eosin[Conclusion]The effective dress dye Active Rose red is much more simply prepared and abundant than eosin.
作者
邵金辉
张敏
唐一通
冯桂花
SHAO Jin-hui;ZHANG Min;TANG Yi-tong;FENG Gui-hua(School of Medicine,Hubei University of Art and Science,Xiangyang 441053,China)
出处
《生物技术》
CAS
2019年第6期562-565,592,共5页
Biotechnology
基金
2017年地方高校国家级大学生创新创业训练计划项目(201710519004)
湖北文理学院博士科研启动基金项目(2020170393)
湖北文理学院教师科研能力培育基金项目
谷城人民医院横向课题
湖北省教育厅科学研究计划指导性项目(B2015144)
作者简介
通讯作者:邵金辉(1970-),男,山东莘县人,博士,副教授,研究方向:组织学与胚胎学,E-mail:jhshao666@163.com。
