摘要
目的观察小鼠成骨细胞(MC3T3-E1)分别在Regesi再生硅和Bio-Oss骨粉材料上的生长情况,分析Regesi再生硅材料、Bio-Oss骨粉对小鼠成骨细胞增殖、成骨分化的影响。方法将实验分为3组,分别为Regesi再生硅实验组、Bio-Oss骨粉实验组和对照组,将两种材料分别放置于24孔板中,对照组为空白,将细胞分别接种在各组24孔板上,培养1、4、7 d时倒置显微镜观察细胞生长情况;培养1、4、7 d时MTT检测细胞的增殖活性;培养7 d时碱性磷酸酶(ALP)试剂盒测定成骨细胞的OD值来分析ALP活性;培养7 d时Rt-PCR方法测定3组细胞的Runx2和Osterix(OSX)基因表达情况。结果显微镜下观察,各组细胞随着时间增加而数量增加;培养7 d时与对照组比较,Regesi再生硅组细胞增殖水平升高(P<0.05),ALP活性、Runx2、OSX mRNA表达水平升高(P<0.05),培养7 d时Bio-Oss骨粉组细胞增殖水平降低(P<0.05),ALP活性、Runx2、OSX mRNA的表达差异无统计学意义(P>0.05)。结论Regesi再生硅能促进MC3T3-E1细胞的增殖、分化水平;Bio-Oss骨粉能减缓细胞增殖,但成骨分化能力与单纯成骨细胞无明显差异。
Objective To observe the growth of mouse osteoblast(MC3T3-E1)on Regesi regeneration silicon and Bio-Oss bone powder,and to analyze the effects of Regesi regeneration silicon materials and Bio-Oss bone powder on mouse osteoblast proliferation and osteogenic differentiation.Methods The experiment was divided into three groups,respectively Regesi regeneration silicon group,Bio-Oss bone powder group and control group.The two materials were placed in 24-well plates,while the control group was blank.Cells were inoculated on 24-well plates in each group,and the cell growth was observed by inverted microscope at 1,4 days,and 7 days of culture;MTT was used to detect cell proliferation activity at 1,4 days,and 7 days of culture;OD value of osteoblasts was determined by alkaline phosphatase(ALP)kit at 7 d culture to analyze ALP activity.Rt-PCR method was used to determine Runx2 and Osterix(OSX)gene expression in 3 groups of cells at 7 days of culture.Results Under the microscope,the number of cells in each group increased with time.The proliferation level of cells in Regesi group increased(P<0.05),and the ALP activity elevated,the expression levels of Runx2 and OSX mRNA increased at 7 days of culture(P<0.05).The proliferation level of cells in the bio-Oss bone meal group was decreased at 7 days of culture(P<0.05),and there was no significant difference in the ALP activity,and the expression levels of Runx2 and OSX mRNA(P>0.05).Conclusion Regesi regenerated silicon can promote the proliferation and differentiation of MC3T3-E1 cells.Bio-Oss bone powder can decrease cell proliferation,while there is no significant difference between osteogenic differentiation ability and osteoblasts alone.
作者
贾琰
张保荣
JIA Yan;ZHANG Baorong(Oral Medicine School,Weifang Medical College,Shandong Province,Weifang 261053,China;Oral Clinic,Aviation General Hospital,Beijing 100012,China)
出处
《中国医药导报》
CAS
2020年第3期13-16,共4页
China Medical Herald
基金
中国科学院动物研究所研究项目(41426040204)
作者简介
贾琰(1994.2-),女,潍坊医学院2017级口腔临床医学专业在读硕士研究生,研究方向:口腔种植学;通讯作者:张保荣(1979.7-),男,博士,副主任医师,研究方向:口腔种植学、牙周学。
