摘要
目的探讨PBK/TOPK在膀胱癌中的表达及其表达对BIU-87细胞株增殖及迁移的影响。方法采用QRT-PCR及Western blot检测3例配对膀胱癌及癌旁正常组织中PBK/TOPK的表达。随后,对膀胱癌BIU-87细胞株中PBK/TOPK的表达分别干扰及过表达,通过CCK-8实验检测细胞的增殖,Transwell实验检测细胞的迁移能力。结果结果一致表明PBK/TOPK在膀胱癌中的表达明显高于癌旁正常组织(P<0.05)。干扰PBK/TOPK表达后,BIU-87细胞的增殖及迁移能力下降(P<0.05);而PBK/TOPK过表达后,BIU-87细胞的增殖及迁移能力增强(P<0.05)。结论PBK/TOPK高表达于膀胱癌,有助于膀胱癌细胞的增殖及迁移。
Objective To investigate the expression of PBK/TOPK and its influence on the proliferation and migration of BIU-87 bladder cancer cell line.Methods QRT-PCR and Western blot were used to detect PBK/TOPK expression in 3 cases of bladder cancer and matched normal tissues.Then,PBK/TOPK expression was knocked down and up-regulated in BIU-87 cell line.CCK-8 assay was used to assess cell proliferation,and Transwell assay was used to evaluate cell migration.Results QRT-PCR and Western blot results demonstrated that PBK/TOPK was highly expressed in bladder cancer tissues compared with those in normal tissues(P<0.05).Meanwhile,PBK/TOPK knockdown significantly inhibited cell proliferation and migration,but PBK/TOPK overexpression significantly promoted cell proliferation and migration(P<0.05).Conclusions PBK/TOPK is highly expressed in bladder cancer,which is contributed to cell proliferation and migration.
作者
贾建民
葛永超
段堃
李岩
赵艳
夏伟
吴凡
Jia Jianmin;Ge Yongchao;Duan Kun;Li Yan;Zhao Yan;Xia Wei;Wu Fan(Department of Laboratory,the Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China;Department of Urology,the Third People's Hospital of Zhengzhou,Zhengzhou 450000,China;Department of Nephrology,the Third People's Hospital of Zhengzhou,Zhengzhou 450000,China)
出处
《国际泌尿系统杂志》
2020年第1期5-8,共4页
International Journal of Urology and Nephrology
关键词
膀胱肿瘤
细胞
培养的
蛋白质丝氨酸苏氨酸激酶
细胞增殖
细胞运动
Urinary Bladder Neoplasms
Cells
Cultured
Protein-Serine-Threonine Kinases
Cell Proliferation
Cell Movement
作者简介
通信作者:葛永超,Email:3262191383@qq.com。
