摘要
目的:优化伊贝母多糖(FPSP)的提取工艺,并对其结构进行研究。方法:采用Box-Behnken设计-响应面法,以FPSP产率为响应值,对FPSP提取工艺中的料液比、提取温度、提取时间进行优化。通过紫外吸收光谱法、傅里叶红外光谱(FTIR)法、气相色谱-质谱联用(GC-MS)法、刚果红染色法、扫描电子显微镜(SEM)观察、X射线衍射(XRD)法及热化学分析等表征手段对FPSP进行结构初探。结果:FPSP提取的最优工艺参数为料液比1∶28(g/mL)、提取温度94℃、提取时间2.5 h,产率为16.25%(n=3),与理论产率(16.58%)的误差为0.33%。FPSP是由物质的量之比为1∶58.02∶0.73的木糖、葡萄糖、半乳糖及微量甘露糖组成,在280 nm波长附近有弱吸收峰,属于α-构型的吡喃多糖,存在三螺旋结构,其表面呈显不规则颗粒堆积而成的网状结构,同时存在晶体与非晶体结构,具有较好的热稳定性。结论:优化后的FPSP提取工艺合理、产率较高,可为伊贝母资源的进一步开发利用提供参考。
OBJECTIVE:To optimize the extraction technology of Fritillaria pallidiflora polysaccharides(FPSP),and to study its structure.METHODS:Using the yield of FPSP as response value,Box-Behnken design-response surface methodology was adopted to optimize solid-liquid ratio,extraction temperature and extraction time of FPSP extraction technology.Structural properties of FPSP was characterized by UV spectrum,FTIR,GC-MS,Congo red staining,SEM,XRD and thermogravimetric analysis.RESULTS:The optimal technology parameters of FPSP were solid-liquid ratio of 1∶28(g/mL),extraction temperature of 94℃,extraction time of 2.5 h;the yield of FPSP was 16.25%(n=3),the error of which to theoretical yield(16.58%)was 0.33%.FPSP contained xylose,glucose and galactose with a molar ratio of 1∶58.02∶0.73,and trace amount of mannose;there was a weak absorption peak near the wavelength of 280 nm;belonged toα-configuration pyranopolysaccharide.FPSP was in triple-helical structure.The surface of FPSP was a network structure formed by irregular particles.FPSP had both crystalline and amorphous structures.FPSP had good thermostability.CONCLUSIONS:The optimized extraction technology of FPSP is reasonable,and has high extraction yield.The research might provide reference for the further development and utilization of F.pallidiflora.
作者
阿衣吐逊·阿布都外力
帕尔哈提·柔孜
高彦华
排合尔丁·木台力甫
热合巴提·努尔夏提
宁慧霞
阿布力米提·伊力
Ayituxun·Abuduwaili;Paerhati·Rouzi;GAO Yanhua;Paiheerding·Mutailifu;Rehebati·Nuerxiati;NING Huixia;Abulimiti·Yili(Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011,China;University of the Chinese Academy of Sciences,Beijing 100049,China;College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China)
出处
《中国药房》
CAS
北大核心
2020年第3期276-281,共6页
China Pharmacy
基金
国家自然科学基金联合基金重点支持项目(No.U1403201)
中科院西部之光-西部青年学者A类项目(No.2017-XBQNXZ-A-004)
关键词
伊贝母多糖
BOX-BEHNKEN设计
响应面法
结构表征
提取工艺
Fritillaria pallidiflora polysaccharide
Box-Behnken design
Response surface methodology
Structural characterization
Extraction technology
作者简介
阿衣吐逊·阿布都外力,博士研究生。研究方向:天然产物的提取分离及活性成分筛选。电话:0991-3835708。E-mail:ayituxunabuduwaili14@mails.ucas.ac.cn;通信作者:阿布力米提·伊力,研究员,博士,博士生导师。研究方向:天然药物物质基础。电话:0991-3835708。E-mail:abu@ms.xjb.ac.cn。
