摘要
目的通过检测粒细胞巨噬细胞集落刺激因子(GM-CSF)对氟尿嘧啶(5-FU)诱导后胃癌细胞凋亡的影响,初步探讨GM-CSF参与胃癌细胞耐药性的相关机制。方法体外培养胃癌SGC7901细胞,MTT法检测5-FU作用于胃癌细胞的半致死剂量;将GM-CSF添加到5-FU处理的胃癌细胞中,MTT法和平板克隆实验检测其对细胞活性和克隆增殖的影响,Annexin V-FITC/PI双染法流式细胞术检测细胞凋亡情况。Western blotting检测凋亡相关蛋白Bax和Bcl-2的表达差异。结果5-FU对胃癌细胞的半致死剂量为(16±0.4)mg/L。5-FU对胃癌细胞的活性和增殖能力有明显的抑制作用,GM-CSF能有效减弱5-FU的抑制效果(P<0.05)。5-FU能显著诱导胃癌细胞凋亡,Bax/Bcl-2比值显著升高,而加入GM-CSF后,5-FU诱导的凋亡被抵抗,Bax/Bcl-2比值升高趋势被抑制(P<0.05)。结论GM-CSF能够促进胃癌细胞增殖并有效抑制5-FU诱导的胃癌细胞凋亡,在增强胃癌细胞化疗耐药性方面具有重要作用。
Objective By detecting the effects of granulocyte macrophage colony stimulating factor(GM-CSF)on the apoptosis of gastric cancer cells induced by fluorouracil(5-FU),the mechanism of GM-CSFinvolved in drug resistance of gastric cancer cells was preliminarily explored.Methods The gastric cancer SGC7901 cells were cultured in vitro,and the semi-lethal dose of 5-FU on gastric cancer cells was detected by MTT assay.GM-CSF was added to 5-FU-treated gastric cancer cells.The effects of cell proliferation and clonal proliferation were detected by MTT and plate cloning assay.Apoptosis was detected by Annexin V-FITC/PI flow cytometry.Western blotting was used to detect the expression differences of apoptosis-related proteins Bax and Bcl-2.Results The semi-lethal dose of 5-FU to gastric cancer cells was(16±0.4)mg/L.5-FU could inhibit the activity and proliferation of gastric cancer cells,which could be effectively weakened by GM-CSF(P<0.05).5-FU induced apoptosis of gastric cancer cells significantly,Bax/Bcl-2 ratio was significantly increased,while GM-CSF could reverse these trend(P<0.05).Conclusion GM-CSF can promote the proliferation of gastric cancer cells and effectively inhibit the apoptosis of gastric cancer cells induced by 5-FU,and plays an important role in enhancing the chemoresistance of gastric cancer cells.
作者
常仕林
何进鹏
陈亚琼
蔡辉
Chang Shilin;He Jinpeng;Chen Yaqiong;Cai Hui(Ningxia Medical University,Yinchuan 750004,China;Laboratory of Space Radiation Biology,Lanzhou Branch of Chinese Academy of Sciences,Lanzhou 730013,China;Medical Office,Gansu Provincial People’s Hospital,Lanzhou 730013,China;Department of General Surgery,Gansu Provincial People’s Hospital,Lanzhou 730013,China)
出处
《中华普通外科学文献（电子版）》
2020年第1期10-13,共4页
Chinese Archives of General Surgery(Electronic Edition)
关键词
粒细胞巨噬细胞集落刺激因子
胃肿瘤
细胞凋亡
抗药性
肿瘤
氟尿嘧啶
Granulocyte-macrophage colony-stimulating factor
Stomach neoplasms
Apoptosis
Drug resistance
neoplasm
Fluorouracil
作者简介
通信作者:蔡辉,Email:caialonteam@163.com。
