摘要
采用一锅法合成了包裹溶菌酶作配体的金纳米颗粒(Lys-AuNPs)的ZIF-8(Lys-AuNPs@ZIF-8)纳米复合材料,用于水溶液中Hg^2+的检测.通过傅里叶红外光谱(FT-IR)、扫描电子显微镜(SEM)、透射电子显微镜(TEM)、粉末X-射线衍射(XRD)、氮气吸脱附等技术对其结构进行表征.该纳米复合材料粒径大小为300~500 nm,具有良好的水溶性、分散性及荧光稳定性.加入Hg^2+后,AuNPs在640 nm处的荧光发射峰荧光强度减弱,而Lys在430 nm处的荧光发射峰荧光强度几乎不变,因此Lys-AuNPs@ZIF-8可作为检测Hg^2+的比率荧光传感器.F 640/F430比值与Hg^2+摩尔浓度在24~450 nmol•L^-1范围内呈良好的线性关系,当信噪比为3时计算出最低检出限为7.5 nmol•L^-1.
In this work,Lys-AuNPs@ZIF-8 Nanocomposites that lysozyme takes as ligand is synthesized by one-pot method,which is used to detect Hg^2+ in aqueous solution.The Lys-AuNPs@ZIF-8 is investigated by FT-IR,SEM,TEM,XRD and N2 adsorption/desorption analysis,etc.The experimental results show that the nanocomposite has a particle size with the diameters of 300~500 nm,and has good water solubility,dispersibility and fluorescence stability.After the addition of Hg^2+ ,the fluorescence intensity of AuNPs emission peak at 640 nm is weakened,while the fluorescence intensity of Lys emission peak at 430 nm is almost unchanged.Therefore,Lys-AuNPs@ZIF-8 can be used as a ratiometric fluorescence sensor for detecting Hg^2+ .The F 640 nm/F 430 nm ratio has a good linear relationship with the Hg^2+ concentration in the range from 24 to 450 nmol•L^-1.The low detection limit based on a 3d/slope is 7.5 nmol•L^-1.
作者
候林丽
张莎莎
毛艳
汪莉
HOU Linli;ZHANG Shasha;MAO Yan;WANG Li(College of Chemistry and Chemical Engineering,Jiangxi Normal University,Nanchang Jiangxi 330022,China;Jiangxi Ji′an Hydrology Bureau,Ji′an Jiangxi 343100,China)
出处
《江西师范大学学报（自然科学版）》
CAS
北大核心
2020年第1期63-70,共8页
Journal of Jiangxi Normal University(Natural Science Edition)
基金
国家自然科学基金(21465015)
江西省水文局青年科技创新基金(SWJJ-KT201805)
水利部鄱阳湖水资源水环境研究中心开放基金(ZXKT201707)资助项目
作者简介
通信作者:汪莉(1975-),女,江西南昌人,教授,博士,博士生导师,主要从事生物纳米传感分析研究.E-mail:lwanggroup@aliyun.com。
