摘要
目的探索紫草素对乙酰胆碱-氯化钙(Ach-CaCl2)诱导的房颤大鼠的保护作用及可能机制。方法采用雄性SD大鼠尾静脉注射Ach-CaCl2混合液建立大鼠房颤模型,分为正常对照组、模型组与紫草素组。其中,紫草素组预先1周灌胃给予紫草素4mg/(kg·d),再与模型组同时给予Ach-CaCl2混合液造模,采用心电图检测、心脏彩超观察、Masson染色、ELISA法、Western blot等方法评价紫草素对房颤大鼠的保护作用。结果心电图结果显示紫草素延长了大鼠房颤诱发时间,彩超结果显示紫草素改善房颤大鼠心脏扩大情况,ELISA结果显示紫草素降低了房颤大鼠血清炎症因子IL-6和TNF-α水平,Western blot法同样表明紫草素抑制了房颤大鼠心房组织的IL-6和TNF-α的蛋白表达。结论紫草素对房颤大鼠具有保护作用。
OBJECTIVE To evaluate the potential therapeutic effects and mechanism of shikonin on atrial fibrillation in vivo.METHODS Male Sprague-Dawley rats were injected with Ach-CaCl2 mixture to make atrial fibrillation model.They were divided into blank group,model group and shikonin group.4 mg/(kg·d)Shikonin was given to the shikonin group by oneweek gavage in advance,Then given Ach-CaCl2 mixture at the same time.The effects of shikonin on atrial fibrillation in rats were evaluated by electrocardiogram,color Doppler ultrasound,Masson staining,ELISA and Western blot.RESULTS Electrocardiogram showed that shikonin prolonged the induction time of atrial fibrillation;color ultrasonographic results showed that shikonin improved heart enlargement in rats with atrial fibrillation;shikonin inhibited serum inflammatory factors IL-6 and TNF-αin rats with atrial fibrillation;Western blot also showed that shikonin inhibited the expression of IL-6 and TNF-α in atrial tissue of atrial fibrillation rats.CONCLUSION Shikonin shows a protective effect on a rat model of atrial fibrillation.
作者
王钥
陈斌
卢悟广
蔡蕊
徐留刚
黄丹
曹鹏
沈建平
周谦
WANG Yue;CHEN Bin;LU Wu-guang;CAI Rui;XU Liu-gang;HUANG Dan;CAO Peng;SHEN Jian-ping;ZHOU Qian(Affiliated Hospital of Integrated Traditional ChinesE-and Western Medicine,Nanjing University of ChinesE-Medicine,Nanjing,210028,China;Nanjing Research InstitutE-for ComprehensivE-Utilization of Wild Plants,Nanjing,210042,China;School of Pharmacy,Nanjing University of ChinesE-Medicine,Nanjing,210023,China)
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2020年第1期78-82,共5页
Journal of Nanjing University of Traditional Chinese Medicine
基金
国家自然科学基金(81873274,81603332)
江苏省自然科学基金(BK20161082)
江苏省研究生科研与实践创新计划(KYCX19_1280)
作者简介
第一作者:王钥,女,硕士研究生,E-mail:1321741020@qq.com;通信作者:沈建平,男,主任医师,教授,主要从事心血管疾病的临床研究工作,E-mail:sjpcn63@163.com;通信作者:周谦,女,副研究员,主要从事心血管疾病的基础研究工作,E-mail:njzhouqian@163.com。
