摘要
研究了CS/GS/GC纳米电化学传感器的制备,建立了一种水产品中隐色孔雀石绿的快速灵敏的检测方法。LMG的循环伏安图表明在0.79、1.18 V左右出现2个氧化峰,在0.98 V左右出现一个还原峰,这表明LMG的电化学氧化两步完成,还原则是一步完成的。优化了纳米电化学传感器的制备及检测条件:修饰材料CS与GS之比为1:2、修饰液用量为5μL、0.15 mol/L LiClO4为电解质、乙腈为溶剂;在此条件下LMG氧化峰电流与其浓度在0.9×10^-7~0.1×10^-7 mol/L具有良好的线性关系,其线性方程:Ip(μA)=54.05 CLMG(μmol/L)+0.686(R^2=0.980),检测限3.99×10^-9mol/L(S/N=3),加标回收率在99.87%~102%。该方法用于水产品中LMG的测定,与其他检测方法相比,具有检测速度快、结果灵敏度高、稳定性好、重复性好、样品处理简单的优势,所以该方法用于水产品中LMG的快速检测是可行的。
The preparation of CS/GS/GC nano-electrochemical sensor was studied, and a rapid and sensitive detection method for leucomalachite green(LMG) in aquatic products was established. The electrochemical behavior of LMG was investigated. The cyclic voltammogram of LMG showed that there were two oxidation peaks at 0.79 V and 1.18 V, and a reduction peak at about 0.98 V, which indicated that its electrochemical oxidation is carried out in two steps, but the electrochemical reduction is completed in one step. The assembly of nano-electrochemical sensors and detection conditions were optimized: the ratio of CS to GS was 1:2, the dosage of modification solution was 5 μL, 0.15 mol/L LiClO4 was electrolyte, and acetonitrile was solvent. At the optimum conditions, the oxidation peak currents of LMG increased linearly with its concentration in the range of 0.9×10^-7~0.1×10^-7 mol/L[Ip(μA)=54.05 CLMG(μmol/L)+0.686, R^2=0.980], with the limit of detection(LOD) as 3.99×10^-9 mol/L(S/N=3). The addition standard recovery of the developed method was 99.87% to 102%. The results of the developed method for determination of LMG in aquatic products agreed with those of HPLC method, at the same time, it has the advantages of convenience, quickness, sensitivity good repeatability and simple sample treatment. So it is practical for the rapid determination of LMG in aquatic products.
作者
尚晶晶
郝雅茹
李书国
SHANG Jingjing;HAO Yaru;LI Shuguo(Hebei University of Science and Technology,Shijiazhuang 050018)
出处
《食品科技》
CAS
北大核心
2019年第11期351-356,共6页
Food Science and Technology
基金
国家自然科学基金项目(20876165)
河北省食品药品安全科技计划项目(ZD2014011)
关键词
隐色孔雀石绿
食品安全
石墨烯
电化学分析
快速检测
leucomalachite green
food safety
graphene
electrochemical analysis
rapid detection
作者简介
尚晶晶(1992—),女,硕士研究生,研究方向为食品安全快速检测;通信作者:李书国。
