摘要
海藻糖是生物体合成的、具有抗逆保护作用的天然二糖,在食品加工与贮藏、微生物发酵过程具有重要作用。该文采用前期获得的专一性海藻糖酶,建立了一种定量测定海藻糖的快速方法。该方法对海藻糖的检测限为0.019 mg/m L,定量限为0.1 mg/m L;海藻糖的线性检测范围为0.1 mg/m L^150.0 mg/m L,回收率为98.80%~101.33%。该方法操作简便快捷、特异性高、重复性好,在菌种改良与食品原料营养筛查等海藻糖快速定量检测中具有良好的应用前景。
Trehalose is a natural disaccharide synthesized by living organisms with stress tolerance and protection.It plays an important role in food processing and storage,as well as in microbial physiology.In this study,a rapid method for quantifying trehalose was developed using a selective trehalase which was obtained previously.The detection limit was 0.019 mg/m L and its quantification limit was 0.1 mg/m L.The linear range of detection was between 0.1 mg/m L and 150.0 mg/m L.The product recovery rate was 98.80%-101.33%.This new method is simple,rapid,with high specificity and reproducible.It has potential applications in quantifying trehalose during strain development and raw material nutrient analysis.
作者
苗佳
王彩喆
牛丹丹
Nokuthula Peace Mchunu
田康明
Suren Singh
Kugenthiren Permaul
王正祥
MIAO Jia;WANG Caizhe;NIU Dandan;Nokuthula Peace MCHUNU;TIAN Kangming;Suren SINGH;Kugenthiren PERMAUL;WANG Zhengxiang(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;College of Chemical Engineering and Materials Science,Tianjin University of Science andTechnology,Tianjin 300457,China;Biotechnology Platform,Agricultural Research Council,Pretoria 0001,South Africa;Department of Biotechnology&Food Technology,Durban University of Technology,Durban 4001,South Africa)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2019年第23期227-231,共5页
Food and Fermentation Industries
基金
国家重点研发计划政府间国际科技创新合作重点专项(2018YFE0100400)
天津市高等学校创新团队建设规划(TD12-5002)
天津市科技计划项目(19YFZCSN00560)
关键词
海藻糖
快速检测
海藻糖酶
trehalose
rapid determination
trehalase
作者简介
第一作者:苗佳,硕士研究生;通讯作者:田康明,博士,E-mail:kangmingtian@tust.edu.cn;通讯作者:王正祥,教授,E-mail:zxwang0519@tust.edu.cn
