摘要
目的探讨颌下腺超声成像在评价干燥综合征动物模型中的作用和价值。方法C57BL/6雌性小鼠建立SS模型,在d 0、21、28和35对小鼠进行唾液量、饮水量和体重等常规指标检测,d 35检测颌下腺指数和病理改变,同时运用Vevo 2100小动物超声成像系统,对小鼠颌下腺部位进行纵切超声检查,将超声观察的结果与常规检测指标进行相关性分析。结果SS小鼠造模d 28出现唾液量明显减少,饮水量显著增多,造模d 35颌下腺指数明显增高,颌下腺发生SS的典型病理改变。超声扫描在造模d 21发现SS组颌下腺轴面面积较正常组明显增高,颌下腺血流信号在d 28和35较正常组明显增多。分析表明,超声观察的结果与常规检测指标具有显著相关性。结论颌下腺的超声成像可能为SS动物模型的评价提供早期线索和半定量指标,在评价SS模型病情变化以及治疗SS的药效学基础研究中具有重要价值。
Aim To evaluate the application of submandibular gland ultrasound in diagnosing mouse model of Sj gren’s syndrome(SS).Methods SS model was established in C57BL/6 female mice.The saliva volume,water intake and body weight of mice were measured on d 0,d 21,d 28 and d 35 after the first immunization respectively,then the index and pathology of submandibular gland were detected on d 35.Meanwhile,ultrasonic examination was performed on the submandibular gland on a Vevo 2100 small animal ultrasound imaging system.The correlations between the ultrasound observations and the clinic manifestations or submandibular gland histology of SS mouse were analyzed.Results The saliva secretion of SS mice began to decrease significantly on d 28 after modeling,the index of submandibular gland increased significantly on d 35,and the typical pathological changes of SS were observed in submandibular gland from model mice.On d 21,it was found that a marked area increment of submandibular gland in axial section of SS mouse compared with that of normal mice.More blood flow signals were detected in submandibular gland of SS mice on d 28 and d 35.The analysis showed an evident correlation between the results of ultrasonic observation and the conventional detection indexes.Conclusions Ultrasonography of submandibular gland is able to provide early clues and sensitive semi-quantitative indexes for evaluating the pathological changes of SS mouse model.It is of great value in dynamically observing the process of SS and thus it is a promising approach for pharmacodynamic studies of anti-SS drugs.
作者
周伟杰
周正炜
陶娟
邰宇
汪丹丹
王珍
郭派派
汪庆童
魏伟
ZHOU Wei-jie;ZHOU Zheng-wei;TAO Juan;TAI Yu;WANG Dan-dan;WANG Zhen;GUO Pai-pai;WANG Qing-tong;WEI Wei(Institute of Clinical Pharmacology,Anhui Medical University,Key Lab of Anti-inflammatory and Immune Medicine,Ministry of Education,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine,Hefei 230032,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2020年第1期140-145,共6页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81202541,81330081)
安徽省杰出青年学者自然科学基金(1808085J28)
安徽高校自然科学研究重点项目(KJ2017A176)
安徽省高校优秀青年人才支持计划项目(gxyqZD2017025)
安徽省留学回国人员创新创业扶持计划
安徽医科大学青年拔尖人才资助计划(2013年)
安徽医科大学中青年学术技术带头人计划
作者简介
周伟杰(1993-),男,硕士生,研究方向:抗炎免疫药理学,E-mail:1010616730@qq.com;通讯作者:汪庆童(1979-),女,博士,教授,硕士生导师,研究方向:抗炎免疫药理学,E-mail:hfwqt727@163.com;通讯作者:魏伟(1960-),男,博士,教授,博士生导师,研究方向:抗炎免疫药理学,E-mail:wwei@ahmu.edu.cn。
