摘要
目的:探究微小RNR-133(miR-133)对肝癌细胞增殖、侵袭和迁移的影响及其作用机制。方法:采用实时定量PCR检测miR-133在肝癌组织或肝癌细胞中的表达。采用Lipofectamine 2000试剂转染miR-133模拟物和miR-133抑制物,随后采用CCK-8和Transwell实验分析人肝癌细胞系HepG2细胞的增殖、侵袭和迁移能力变化。通过PicTar软件预测miR-133的靶基因,并进一步通过双荧光素酶报告实验验证其结合能力。最后利用蛋白质印迹法检测蛋白表达变化。结果:miR-133在肝癌组织和肝癌细胞中的表达显著降低。miR-133能够抑制HepG2细胞的增殖、迁移及侵袭能力。此外,miR-133能够靶向调控表皮生长因子受体(EGFR)的表达,敲低EGFR防止了miR-133下调对肿瘤细胞增殖、侵袭及迁移能力的促进作用。结论:miR-133通过影响EGFR表达而抑制肝癌细胞的增殖、侵袭及迁移。
Objective:To investigate the effect and mechanism of invasion and migration of miR-133 on hepatocellular carcinoma cells.Methods:Real-time PCR was used to detect the expression of miR-133 in tumor tissues or cells.MiR-133 mimic and inhibitor were transfected with Lipofectamine 2000 reagent,then CCK-8 and Transwell analysis were used to detect the potential of proliferation,invasion and migration of human liver cancer cell line HepG2.The target of miR-133 was predicted by PicTar software and luciferase reporter assay was used to verify the binding ability between them.The level of protein was detected by Western blot experiments.Results:The expression of miR-133 in hepatocellular carcinoma tissues and hepatocellular carcinoma cells decreased significantly.MiR-133 inhibited the proliferation,invasion and migration of HepG2 cells.Moreover,miR-133 targeted and regulated epidermal growth factor receptor(EGFR).EGFR knockdown prevented miR-133 downregulation induced hepatoma carcinoma cells proliferation and invasion.Conclusion:MiR-133 inhibits the proliferation,invasion and migration of hepatocellular carcinoma cells by affecting the expression of EGFR.
作者
胡东辉
黄橘村
张建军
HU Donghui;HUANG Jucun;ZHANG Jianjun(Hepatology Department of Integrated Traditional Chinese and Western Medicine,the Third People s Hospital of Hubei Province,Wuhan 430032,China)
出处
《东南大学学报（医学版）》
CAS
2019年第6期1044-1049,共6页
Journal of Southeast University(Medical Science Edition)
关键词
微小RNR-133
表皮生长因子受体
肝癌
增殖
侵袭
miR-133
epidermal growth factor receptor
hapertocellular carcinoma
proliferation
invasion
作者简介
胡东辉(1984),男,湖北武汉人,主治医师。E-mail:231128112@qq.com;通信作者:张建军,E-mail:284626814@qq.com。
