摘要
目的计算洁净室中沉降菌测量的不确定度。方法建立数学模型,评估和量化沉降菌测定过程中可能产生影响的各个不确定度分量,分别计算A、B类各不确定度分量值、合成标准不确定度、扩展不确定度。结果沉降菌的菌落数为4个/皿时,其合成标准不确定度为0.861个/皿,取包含因子k=2(置信概率为95%),沉降菌的扩展不确定度为U=u_c×k=0.861×2≈2个/皿;洁净区沉降菌结果为(4±2)个/皿,k=2。结论对于洁净室沉降菌的不确定度评定,其中A类不确定度这一分量占比最大,B类不确定度各个分量值均很小,所以进行沉降菌的不确定度评定时主要是以A类不确定度为主,B类不确定度所带来的影响可以忽略不计。
Objective To evaluate the uncertainty of measurement of settling microbe in clean room.Methods The mathematical model was established to evaluate and quantify the various uncertainty components that might affect the sedimentation process,and the uncertainty component values of A and B,the synthetic standard uncertainty,and the extended uncertainty were calculated.Results When the colony number of settling microbe was 4/plate,the standard uncertainty of synthesis was 0.861/plate.Taking the inclusion factor k=2(95%confidence level),the extended uncertainty of settling microbe was U=u_c×k=0.861×2≈2/plate,and the result of settling microbe in the clean area was(4±2)/plate,k=2.Conclusion For the uncertainty assessment of clean room settling bacteria,the component of class A uncertainty accounts for the largest proportion,and the component of class B uncertainty is small.Therefore,the uncertainty of the settlement bacteria is mainly based on the class A uncertainty,and the impact of the class B uncertainty is negligible.
作者
李诗华
李建新
刘长富
蔡伟江
LI Shi-Hua;LI Jian-Xin;LIU Chang-Fu;CAI Wei-Jiang(By-Health Co.,Ltd.,Zhuhai 519040,China)
出处
《食品安全质量检测学报》
CAS
2019年第23期8115-8118,共4页
Journal of Food Safety and Quality
关键词
沉降菌
测量不确定度
洁净室
settling microbe
measurement uncertainty
clean room
作者简介
李诗华,主要研究方向为膳食营养补充剂的质量检测。E-mail:921865587@qq.com;通讯作者:李建新,主要研究方向为膳食营养补充剂的质量检测。E-mail:815455530@qq.com。
