摘要
目的探讨联合有氧-抗阻运动(联合运动)对2型糖尿病小鼠骨髓内皮祖细胞(EPCs)血管生成能力的影响及其可能机制。方法将20只2型糖尿病db/db小鼠随机均分为联合运动组以及对照组。对照组小鼠不进行任何运动干预,联合运动组采取有氧运动与抗阻运动相交替方式进行训练。8周后分离培养小鼠骨髓EPCs,采用密度梯度离心法分离获得单个核细胞后,以EGM-2MV培养基诱导其分化获取EPCs,通过双荧光染色法鉴定EPCs。将联合运动组以及对照组细胞分别分为联合运动组及联合运动+抑制剂组、对照组及对照+抑制剂组,使用磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(AKT)特异性抑制剂LY294002干预各抑制剂组细胞。观察各组EPCs的体外血管生成功能,并检测各组EPCs的AKT、p-AKT蛋白及PI3K、AKT的mRNA表达情况。结果联合运动组EPCs的主干长度和节点均长于或多于对照组及联合运动+抑制剂组(均P<0.05)。联合运动组PI3K、AKT的mRNA表达水平均高于对照组及联合运动+抑制剂组(均P<0.05)。联合运动组的AKT、p-AKT蛋白相对表达水平高于对照组及联合运动+抑制剂组(均P<0.05)。结论联合运动能有效提高2型糖尿病小鼠EPCs血管生成能力,其机制可能与上调AKT蛋白表达有关。
Objective To explore the effect of combined aerobic and resistant exercise(combined exercise) on angiogenesis function of bone marrow endothelial progenitor cells(EPCs) in type 2 diabetic mice and its possible mechanism.Methods Twenty type 2 diabetic db/db mice were randomly and equally divided into combined exercise group and control group.No exercise intervention was conducted in the control group,and the combined exercise group was trained by aerobic and resistant exercise alternately.After 8 weeks,the EPCs were cultured after being isolated from bone marrow,and the mononuclear cells were isolated and obtained by density gradient centrifugation,then the cells were differentiated into EPCs by induction using EGM-2 MV culture medium,and the EPCs were identified by double fluorescence staining.The cells of the combined exercise were divided into combined exercise group and combined exercise + inhibitor group,and the cells of the control groups into control group and control + inhibitor group;the cells of each inhibitor group were intervened by phosphatidylinositol 3-kinase(PI3 K)/protein kinase B(AKT) specific inhibitors LY294002.The angiogenesis function in vitro of the EPCs in each group was observed;besides,the expressions of AKT and phosphorylated PI3 K(p-PI3 K) proteins as well as PI3 K and AKT mRNAs were detected in the EPCs of each group.Results The EPCs in the combined exercise group obtained longer trunk length and more nodes compared to the control group and the combined exercise + inhibitor group(all P<0.05).The combined exercise group yielded higher relative expression levels of PI3 K and AKT mRNAs compared to the control group and the combined exercise + inhibitor group(all P<0.05).The combined exercise group had higher relative expression levels of AKT and p-PI3 K proteins compared to the control group and the combined exercise + inhibitor group(all P<0.05).Conclusion Combined exercise can effectively improve the angiogenesis ability of EPCs in type 2 diabetic mice,and its mechanism may be closely related to the up-regulation of the expressions of AKT protein.
作者
黄燕凤
翟露
刘玉花
马翠
戴霞
HUANG Yan-feng;ZHAI Lu;LIU Yu-hua;MA Cui;DAI Xia(Department of Nursing,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)
出处
《广西医学》
CAS
2019年第22期2887-2891,2908,共6页
Guangxi Medical Journal
基金
国家自然科学基金(81660147)
广西南宁市青秀区科技计划(2018027)
关键词
2型糖尿病
内皮祖细胞
联合有氧-抗阻运动
血管生成能力
磷脂酰肌醇3-激酶
蛋白激酶B
小鼠
Type 2 diabetes
Endothelial progenitor cells
Combined aerobic and resistant exercise
Angiogenesis ability
Phosphatidylinositol 3-kinase
Protein kinase B
Mice
作者简介
黄燕凤(1995-),女,在读硕士研究生,研究方向:内科护理;通信作者:戴霞(1965-),女,本科,主任护师,研究方向:糖尿病管理与内科护理,电子邮箱:2655947220@qq.com。
