摘要
目的探讨多环芳烃(polycyclic aromatic hydrocarbons,PAHs)暴露对大鼠学习记忆的影响及可能机制。方法健康雄性SD大鼠40只,随机分为4组:植物油组(对照组)、低剂量组(PAHs 3.2mg/kg)、中剂量组(PAHs 8.0mg/kg)、高剂量组(PAHs 20.0mg/kg),根据大鼠体质量腹腔注射染毒共60d。染毒结束后用Morris水迷宫检测大鼠的学习记忆能力,旷场实验测定大鼠对新环境的适应能力,相关试剂盒检测大鼠脑组织的脂质过氧化水平和神经递质含量。结果与对照组相比,随着染毒剂量的增加,大鼠平台象限滞留时间及站立时间缩短,差异有统计学意义(P<0.05),染毒组大鼠活性氧含量升高,谷氨酸含量降低,差异均有统计学意义(P<0.05)。结论PAHs暴露影响大鼠的空间学习记忆能力,并引起大鼠脑内活性氧含量升高及谷氨酸含量降低,这可能是多环芳烃暴露影响大鼠学习记忆能力下降的机制之一。
Objective To explore the effects and possible mechanisms of polycyclic aromatic hydrocarbons(PAHs)on learning and memory in rats.Methods 40healthy male SD rats were randomly divided into 4groups:control group(vegetable oil),low dose PAHs group(3.2mg/kg),medium dose PAHs group(8.0mg/kg)and high dose PAHs group(20.0mg/kg).Rats were exposed to different dose of PAHs by intraperitioneal injection once in two days for 60days.Morris water maze and open-field tests were used to measure the capability of learning and memory and the adaptability to novel environment.Related assay kits were used to detect lipid peroxidation and neurotransmitters content in rat brain.Results Compared to control mice,time in zone and rearing time of mice exposed to PHAs were significantly shortened(P<0.05).Levels of reactive oxygen species(ROS)in exposed mice were significantly higher than that in controls(P<0.05),while levels of glutamic acid were significantly lower(P<0.05).Conclusions PAHs exposure affects spatial learning and memory ability in rats by increasing ROS level and reducing glutamic acid in the brain.
作者
杨柳叶
张斌
康辉
郭建勇
陈佳慧
王芳
YANG Liu-ye;ZHANG Bin;KANG Hui;GUO Jian-yong;CHEN Jia-hui;WANG Fang(School of Public Health,Shanxi Medical University,Taiyuan,Shanxi 030001,China)
出处
《中国预防医学杂志》
CAS
CSCD
2019年第10期893-896,共4页
Chinese Preventive Medicine
基金
国家自然科学基金资助项目(81502795)
关键词
多环芳烃
SD大鼠
学习记忆
脂质过氧化
神经逆质
神经毒性
旷场实验
Polycyclic aromatic hydrocarbons
SD rats
Learning and memory
Lipid peroxiadation
Neurotransmitters
Neurotoxicity
Open-field test
作者简介
杨柳叶,硕士研究生,主要从事职业流行病学研究;通信作者:王芳,E-mail:wfang@sxmu.edu.cn。
