摘要
目的:探讨肠道微生态平衡抗非小细胞肺癌作用的免疫机制。方法:建立C57BL/6J小鼠Lewis肺癌模型72只,根据腹腔注射药物不同分为对照组、顺铂组、顺铂/抗生素组、顺铂/乳酸杆菌组,每组18只。记录各组小鼠肿瘤体积、存活时间;采用Western Blot法检测瘤体VEGFA、Ras、CDKNIB、Bax蛋白表达,EILSA检测血清IL-6、IL-10、TNF-α、IFN-γ水平,PCR检测小鼠肠道菌群数量。结果:与对照组相比,其他组小鼠的肿瘤生长均受到明显抑制。其中,以顺铂/乳酸杆菌组抑瘤作用最明显,顺铂/抗生素组抑制效果最差。与顺铂/乳酸杆菌组相比,顺铂组与顺铂/抗生素组的生存时间明显更短。与对照组相比,顺铂组小鼠致癌基因VEGFA、Ras的表达水平分别降低了47.9%、33.2%,抑癌基因Bax、CDKNIB的表达水平分别上调了121.7%、128.9%。与顺铂组对比,抗生素减弱了顺铂对VEGFA的下调及对CDKNIB、Bax的上调作用。相反地,乳酸杆菌明显增强了顺铂对VEGFA水平的下调及对CDKNIB的上调作用。ELISA检测结果发现,与顺铂/抗生素组比较,顺铂/乳酸杆菌组IL-6、IFN-γ水平显著升高,IL-10水平显著降低(P<0.05),但TNF-α无明显差异(P>0.05)。与对照组相比,顺铂组小鼠4种菌群数量均呈现下降趋势,但差异无统计学意义(P>0.05),顺铂/抗生素组4种菌群数量下降最为明显(P<0.05),顺铂/乳酸杆菌组菌群数量与对照组基本接近。结论:肠道微生态平衡有助于抗肺癌作用,能增强顺铂的抗肿瘤效应。
Objective:To explore the immune mechanism of intestinal microecological balance in the treatment of non-small cell lung cancer.Methods:72 mice with Lewis lung cancer were divided into control group,cisplatin group,cisplatin/antibiotic group and cisplatin/Lactobacillus group.The tumor volume and survival time of each group were recorded,and the expression of related oncoproteins and serum cytokines were detected.Results:Compared with the control group,tumor growth in other groups was significantly inhibited.Compared with cisplatin/Lactobacillus group,the survival time of cisplatin group and cisplatin/antibiotic group was significantly shorter.Compared with the control group,the expression level of oncogene VEGFA and Ras in cisplatin group decreased by 47.9%and 33.2%respectively,and the expression level of Bax and CDKNIB of tumor suppressor gene was increased with 121.7%and 128.9%respectively.The results of ELISA detection showed that compared with cisplatin/antibiotic group,the level of IL-6 and IFN-gamma in cisplatin/Lactobacillus group increased significantly,and the level of IL-10 decreased significantly,but there was no significant difference in TNF-alpha.Compared with the control group,the number of 4 bacteria groups in cisplatin group showed a decreasing trend,and the number of 4 species of cisplatin/antibiotic group decreased most significantly,and the number of cisplatin/Lactobacillus group was almost close to that of the control group.Conclusion:Intestinal microecological balance can help lung cancer and enhance the antitumor effect of cisplatin.
作者
耿志广
王永锋
Geng Zhiguang;Wang Yongfeng(Department of Respiratory Medicine,People's Hospital of Yishui County,Shandong Linyi 276400,China;Department of Respiratory Medicine,Linyi Central Hospital,Shandong Linyi 276400,China)
出处
《现代肿瘤医学》
CAS
2020年第1期13-16,共4页
Journal of Modern Oncology
关键词
非小细胞肺癌
乳酸杆菌
顺铂
non-small cell lung cancer
Lactobacillus
cisplatin
作者简介
耿志广(1963-),男,山东沂水人,副主任医师,主要从事肺癌临床研究;通讯作者:王永锋(1970-),男,山东人,硕士,副主任医师,主要从事呼吸内科临床研究。
