摘要
为探究外源p-香豆酸(p-coumaric acid,p-CA)处理对采后桃果实冷害和相关机制的影响,本实验以‘沙红’桃果实为试材,研究了0、0.5、1.0 mmol/L p-CA浸泡处理对采后桃果实于4℃贮藏期间冷害指数和丙二醛含量的影响,并得出最优浓度(0.5 mmol/L),在此条件下继续研究其对桃果实苯丙烷代谢和抗氧化能力的影响。结果显示:p-CA处理延缓了桃果实冷害的发生和丙二醛的生成;p-CA处理激活了莽草酸脱氢酶、苯丙氨酸脱氨酶、肉桂酸-4-羟化酶和4-香豆酸辅酶A连接酶活力,并抑制了多酚氧化酶及过氧化氢酶活力,这使桃果实中总酚含量增加,进而提高了清除1,1-二苯基-2-三硝基苯肼自由基、羟自由基、O2-的能力。以上结果表明p-CA处理对桃果实低温贮藏期间冷害发生的延缓作用与其对苯丙烷代谢和抗氧化能力的调控有关。
In order to explore the effect and underlying mechanism of exogenous p-coumaric acid(p-CA)on chilling injury(CI)in postharvest peach fruit,‘Shahong’peach fruit were immersed in 0,0.5 and 1 mmol/L p-CA to investigate the effect of p-CA on CI index and malondialdehyde(MDA)content during subsequent storage at 4℃.It turned out that 0.5 mmol/L p-CA was selected as the optimal treatment concentration.In this case,we further studied the effect of p-CA treatment on phenylpropanoid metabolism and antioxidant capacity in postharvest peach fruit.The results showed that p-CA treatment significantly delayed the development of CI and the generation of MDA in peach fruit.p-CA treatment stimulated the activity of shikimate dehydrogenase,phenylalanine ammonia lyase,cinnamate-4-hydroxylase and 4-coumarate coenzyme A ligase,but inhibited polyphenol oxidase and peroxidase activities,which contributed to higher total phenolic content in peach fruit and thus enhanced 1,1-diphenyl-2-picrylhydrazyl(DPPH),hydroxyl and superoxide anion radical scavenging capacity.This study indicated that the alleviating effect of p-CA on CI was associated with its ability to regulate phenylpropanoid metabolism and antioxidant capacity in peach fruit.
作者
李姚瑶
曹毛毛
王小璐
苏金龙
高慧
LI Yaoyao;CAO Maomao;WANG Xiaolu;SU Jinlong;GAO Hui(College of Food Science and Technology,Northwest University,Xi’an 710069,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第23期227-232,共6页
Food Science
基金
国家自然科学基金青年科学基金项目(31401550)
关键词
p-香豆酸
桃果实
冷害
苯丙烷代谢
抗氧化能力
p-coumaric
peach fruit
chilling injury
phenylpropanoid metabolism
antioxidant capacity
作者简介
第一作者:李姚瑶(1994-)(ORCID:0000-0003-0254-950X),女,硕士研究生,研究方向为果蔬贮藏与加工。E-mail:2780460998@qq.com;通信作者:高慧(1977-)(ORCID:0000-0002-0913-3040),女,副教授,博士,研究方向为果蔬贮藏与加工。E-mail:gaohui0815@163.com。
