摘要
以石柱参近缘种野生山参的人参皂苷合成酶基因SS为模板,利用NCBI中的primer-BLAST设计特异性引物,应用PCR技术从石柱参叶片基因组DNA克隆了SS基因。序列分析显示,克隆的基因片段为1285 bp,能够编码25个氨基酸以上的ORF(Open Reading Frame)有10个,其中最长能够编码295个氨基酸。NCBI序列对比显示,该基因与人参的人参皂苷合成酶基因SS的同源性为97.05%,与西洋参的人参皂苷合成酶基因SS的同源性为96.63%。这些数据表明,从石柱参克隆的基因为其人参皂苷合成酶基因SS。
Specific primers to the ginsenoside synthase-encoding gene(SS)of wild ginseng,a related species of Shizhu Panax ginseng,were designed using primer-BLAST in NCBI.The synthesized primers were used to clone the SS from the genomic DNA of Shizhu Panax ginseng leaves by PCR,and a fragment of 1285 bp was obtained.Sequence analysis showed that the cloned gene contained 10 ORFs(Open Reading Frame),each capable of encoding at least 25 amino acids,among which the greatest capacity could encode 295 amino acids.Sequence alignment in NCBI demonstrated that this gene had 97.05% homology with the SS sequence of Panax ginseng,and 96.63% homology with Panax quinquefolius.Based on these data,it is proposed that the gene cloned from Shizhu Panax ginseng using the wild ginseng-specific primers is the ginsenoside synthase-encoding gene.
作者
王丹丹
孙英新
WANG Dan-dan;SUN Ying-xin(College of Agriculture,Eastern Liaoning University,Dandong 118003,China)
出处
《辽东学院学报（自然科学版）》
CAS
2019年第4期265-269,共5页
Journal of Eastern Liaoning University:Natural Science Edition
基金
中央财政专项课题:生物产业技术研发平台——满族道地药材种植基地建设
丹东市2016科技攻关项目(2016KJ001)
辽东学院科研基金项目(20175QN050)
作者简介
王丹丹(1982—),女,辽宁丹东人,硕士,讲师,研究方向:分子生物学。
