摘要
目的:通过观察芍药甘草汤及其拆方对超高温环境大鼠心肌、下丘脑瞬时感受器电位香草酸受体3(TRPV3)和瞬时感受器电位香草酸受体4(TRPV4)表达的影响,探讨芍药甘草汤及其拆方抗高温的机制。方法:SPF级SD雄性大鼠75只,适应饲养1周后,按体质量随机分为5组,分别为常温正常组,高温正常组,芍药甘草汤组(2.16 g·kg^-1),单味白芍组(1.08 g·kg^-1)和单味甘草组(1.08 g·kg^-1),除常温正常组外,均饲养于人工气候箱,各给药组按相应剂量连续给药13 d,常温正常组和高温正常组均给予同体积的纯净水;通过蛋白免疫印迹法(Western blot)和实时荧光定量聚合酶链式反应(Realtime PCR)测定各组心肌、下丘脑TRPV3,TRPV4蛋白和m RNA的表达情况。结果:与常温正常组比较,高温正常组大鼠心肌TRPV3蛋白表达显著上升(P<0.01);心肌TRPV4 m RNA表达明显下降(P<0.05);下丘脑TRPV3蛋白表达显著上升(P<0.01);下丘脑TRPV3 m RNA表达明显下降(P<0.05)。与高温正常组比较,单味白芍组大鼠心肌TRPV3,TRPV4蛋白表达显著下降(P<0.01);芍药甘草汤组与单味甘草组大鼠心肌TRPV4蛋白表达明显下降(P<0.05);单味白芍组大鼠心肌TRPV4m RNA表达明显上升(P<0.05)。结论:大鼠心肌、下丘脑TRPV3,TRPV4通道在高温环境下被激活,芍药甘草汤及其拆方抗高温机制与调节大鼠心肌、下丘脑TRPV3,TRPV4通道蛋白及m RNA表达有关。
Objective:To observe the effect of Shaoyao Gancaotang and its disassembled prescriptions on the expressions of transient receptor potential vanilloid 3(TRPV3)and transient receptor potential vanilloid 4(TRPV4)in myocardium and hypothalamus of rats in the ultra-high temperature environment,in order to explore the mechanism of Shaoyao Gancaotang and its disassembled prescriptions against the high temperature.Method:Seventy five male SPF-grade SD rats were randomly divided into 5 groups according to their body weight,namely normal control group,high temperature normal control group,Shaoyao Gancaotang group(2.16 g·kg^-1),single Paeoniae Radix Alba(PRA)group(1.08 g·kg^-1)and single Glycyrrhizae Radix et Rhizoma(GRR)group(1.08 g·kg^-1).Except normal control group,the remaining groups were fed in artificial climate boxes for 13 days.The normal control group and the high temperature normal control group were given the same volume of pure water.Western blot and quantitative real-time fluorescence polymerase chain reaction(Real-time PCR)were used to determine expressions of TRPV3,TRPV4 protein and m RNA in myocardium and hypothalamus of each group.Result:Compared with the normothermic control group,the expressions of TRPV3 protein,TRPV4 m RNA,TRPV3 protein and TRPV3 m RNA in the myocardium of the hyperthermic control group were significantly higher than those of the normal control group(P<0.05),and the expression of TRPV3 protein in the hypothalamus was significantly higher than that of the normal control group(P<0.05).Compared with the normal control group,the expressions of TRPV3,TRPV4 proteins in myocardium of rats in PRA group were significantly lower than those in the control group,and the expression of TRPV4 protein in myocardium of rats in Shaoyao Gancaotang group and GRR group was significantly lower than that in control group.The expression of TRPV4 m RNA in myocardium of rats in PRA group was significantly increased(P<0.05).Conclusion:TRPV3,TRPV4 channels in rat myocardium and hypothalamus were activated in the high temperature environment.The anti-hyperthermic mechanism of Shaoyao Gancaotang and its disassembled prescriptions was related to the regulation of TRPV3,TRPV4 channel protein and m RNA expressions in rat myocardium and hypothalamus.
作者
姚凤云
王琳
刘春花
王非云
刘超
姜劼琳
王炳志
YAO Feng-yun;WANG Lin;LIU Chun-hua;WANG Fei-yun;LIU Chao;JIANG Jie-lin;WANG Bing-zhi(College of Traditional Chinese Medicine(TCM),Jiangxi University of TCM,Nanchang 330004,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第24期9-14,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81503489)
江西省卫计委中医药科技计划项目(2016B016)
江西省百人远航工程项目(赣科协字【2016】120号)
作者简介
第一作者:姚凤云,博士,教授,从事方剂配伍规律与现代研究,Tel:0791-87118923,E-mail:hrbyaoyao2000@163.com;通信作者:王炳志,高级实验师,从事复方药理学研究,Tel:0791-87118626,E-mail:wbzh1123@163.com。
