摘要
沙门氏菌是一种常见的食源性人畜共患肠道致病菌,兼性胞内致病菌。山东省某养驴场频发流产现象且无明显征兆,本研究对驴流产胎儿内脏及头部感染细菌进行分离,并对分离株进行鉴定及耐药、毒力分析。结果表明,获得的7株分离株均为沙门氏菌,分别命名为SL1-SL7,其中SL1、SL2、SL3、SL5、SL6与鼠伤寒沙门氏菌的同源性分别为100%、99.93%、99.93%、99.79%、99.57%;SL4与爱丁堡沙门氏菌、汤姆逊沙门氏菌的同源性均为99.79%;SL7与阿邦尼沙门氏菌、爱丁堡沙门氏菌、汤姆逊沙门氏菌同源性分别为99.72%、99.72%、99.65%;耐药表型结果显示7株沙门氏菌对本试验中所有药物均比较敏感;毒力基因筛选结果显示,除SL1、SL2、SL3、SL7毒力岛SPI-4上的ssb毒力基因未检出外,7株分离菌均具有沙门氏菌典型毒力基因;7株沙门氏菌对小鼠的致病性较弱,小鼠喂食菌液后未出现死亡现象。
Salmonella is a common foodborne zoonotic enteric pathogen and facultative intracellular pathogen.There was no obvious sign of frequent abortion in a donkey factory in Shandong Province.In this study,the infection bacteria in viscera and head were isolated,and identifying the isolates and analyzing their drug resistance and virulence.The results showed that all the 7 isolates identified were Salmonella and named as SL1-SL7.The homology rates of SL1,SL2,SL3,SL5 and SL6 with Salmonella typhimurium were 100%,99.93%,99.93%,99.79%and 99.57%,respectively.The homology rates of SL4 with Salmonella edinburgh and Salmonella thomson were 99.79%.The homology rates of SL7 with Salmonella arbonini,Salmonella edinburgh and Salmonella thomson were 99.72%,99.72%and 99.65%,respectively.The results of drug-resistant phenotype showed that 7 strains of Salmonella were sensitive to all the drugs in this study.The results of virulence gene screening showed that all the 7 isolates had typical virulence genes of Salmonella except the ssb virulence genes on SPI-4 of SL1,SL2,SL3 and SL7 virulence islands.The 7 strains of Salmonella were less pathogenic to mice,and the mice did not die after feeding the bacteria solution.
作者
孙阳阳
杨美
解晓莉
程凯慧
张亮
楚会萌
任亚初
杨宏军
Sun Yangyang;Yang Mei;Xie Xiaoli;Cheng Kaihui;Zhang Liang;Chu Huimeng;Ren Yachu;Yang Hongjun(Dairy Cattle Research Center,Shandong Academy of Agricultural Sciences,Jinan 250131,China)
出处
《山东农业科学》
2019年第11期143-148,共6页
Shandong Agricultural Sciences
基金
“十三五”国家重点研发计划项目“牛羊重要疫病诊断与检测新技术研究”(2016YFD0500900)
现代农业(奶牛)产业技术体系科学家岗位项目(CARS-36)
山东省农业科学院农业科技创新工程项目“主要畜禽疫病监测和预警”(CXGC2016A10)
关键词
沙门氏菌
驴
分离鉴定
耐药分析
毒力基因
Salmonella
Donkey
Separation and identification
Resistance analysis
Virulence gene
作者简介
孙阳阳(1989-),女,山东淄博人,硕士,助理研究员,研究方向:分子生物学。E-mail:13688639022@163.com;通讯作者:杨宏军,男,山东济宁人,博士,研究员,研究方向:分子生物学。E-mail:longfei1997@sina.com。
