摘要
【目的】研究不同植物生长调节剂配比处理对德克萨斯组铃铛铁线莲打破休眠及胚胎挽救的影响,解决德克萨斯铃铛铁线莲萌发时间过长,高温胁迫引起种子发育中途败育的问题,为提高育种效率,缩短育种周期提供理论依据与指导。【方法】以德克萨斯组铃铛铁线莲‘王梦’为材料,设计12种不同浓度6-BA、NAA的正交试验筛选铃铛铁线莲胚胎挽救最适植物生长调节剂浓度,讨论椰乳作为培养添加物对胚胎挽救的促进作用;比较低温、变温及不同浓度GA、6-BA对打破铃铛铁线莲种子休眠的影响。【结果】将授粉后45 d的杂交铃铛铁线莲种子离体培养,MS基本培养基添加0.5 mg·L^-1 6-BA和0.05 mg·L^-1 NAA可以使铁线莲胚胎正常发育成熟的成功率达到47%,发芽率为54%;在此基础上培养基中再添加10%的椰乳,种子发育成熟率和发芽率均显著提高到62%。低温处理和光培养25℃/暗培养15℃的温度可以促进铃铛铁线莲种子发芽,配合50 mg·L^-1 GA或30 mg·L^-1 6-BA处理打破休眠更加显著。【结论】铁线莲胚可以通过离体挽救解决高温带来的败育问题;植物生长调节剂处理可以显著打破种子休眠,促进萌发。
【Objective】 To seek means for improvement on crossbreeding the appealing, long flowering, widely adaptable bell clematis by overcoming the long dormancy and high embryo abortion in seed germination.【Method】 Seeds of Dream King clematis(Clematis texensis) collected 45 d after pollination were cultured in vitro on various media to optimize the conditions for embryo rescue and dormancy breaking. An orthogonal experiment of 12 treatments consisting of additions of 6-BA and/or NAA in various concentrations as well as exposures to temperatures and daylight was conducted to observe the resulting seed germination and seedling survival.【Result】 The addition of 0.5 mg·L^-1 6-BA and 0.05 mg·L^-1 NAA to the MS basic medium improved the seed germination rate to 54%, with 47% of the seedlings reached maturation. When 10% of coconut milk was added to the medium, both seed germination and seedling survival rate were significantly raised to reach62%. Exposure of the seeds to low temperature and day 25℃/night 15℃ promoted the germination. And, the presence of either50 mg·L^-1 GA or 30 mg·L^-1 6-BA in the medium facilitated breaking the seed dormancy.【Conclusion】 Embryos of clematis could be rescued in vitro to mitigate premature abortion caused by high temperature. The growth hormone addition could significantly shorten dormancy and promote germination of the bell clematis seeds.
作者
孙瑞琦
余伟军
曾黎辉
SUN Rui-qi;YU Wei-jun;ZENG Li-hui(Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Fujian Forestry Vocational&Technical College,Nanping,Fujian 353000,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2019年第9期1040-1046,共7页
Fujian Journal of Agricultural Sciences
关键词
铃铛铁线莲
杂交
打破休眠
胚胎挽救
bell clematis
hybrid
seed dormancy and seed gernimation
embryo rescue
作者简介
孙瑞琦(1994-),男,硕士,研究方向:花卉与景观园艺(E-mail:308601900@qq.com);通讯作者:曾黎辉(1973-),女,博士,教授,研究方向:花卉基因工程、花色调控(E-mail:125865014@qq.com)。
