摘要
为了探求杉木叶片多酚提取工艺,进一步开发利用杉木资源,采用超声波提取法提取杉木叶片多酚,考察提取时间、乙醇浓度、提取温度、液料比、超声波功率等因素对杉木叶片多酚提取量的影响,并根据响应面Box-Benhnken原理,构建预测模型,对提取工艺进行进一步优化.结果表明:预测最佳工艺条件为提取时间35 min,液料比110:1,乙醇浓度61.50%,提取温度50℃,实测多酚提取量62.72 mg·g^-1;最终优化工艺为提取时间35 min,液料比110:1,乙醇浓度60%,提取温度50℃,此条件下多酚提取量62.92 mg·g^-1.
To optimize the extraction technique of polyphenols from Chinese fir leaves,ultrasonic extraction was applied to Chinese fir leaves,and the optimal operative conditions,which included extraction time and temperature,ethanol concentration,liquid-to-material ratio,ultrasonic power were evaluated by models predicted by response surface Box-Benhnken principle.The results showed that the optimal process conditions were to use 61.50%ethanol and set liquid-to-material ratio at 110:1,then extract ingredients for 35 min under 50℃,yielding an optimal polyphenol at 62.72 mg·g^-1.And these conditions and yield were further comfirmed by verification test.
作者
张健
吕蒙蒙
谢亚聪
武忆寒
林思祖
陈宇
ZHANG Jian;LU Mengmeng;XIE Yacong;WU Yihan;LIN Sizu;CHEN Yu(College of Forestry,Fujian Agriculture and Forestry University;Chinese Fir Engineering Technology Center of National Forestry and Grassland Administration,Fuzhou,Fujian 350002,China)
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2019年第6期765-770,共6页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家林业和草原局杉木工程技术研究中心平台建设(ptjh13002)
关键词
杉木叶片
超声波提取法
响应面法
多酚
Chinese fir leaves
ultrasonic extraction method
response surface method
polyphenol
作者简介
张健(1993-),男.研究方向:森林培育.Email:1053238672@qq.com;通信作者:陈宇(1983-),男.研究方向:林木遗传育种与森林培育.Email:28811852@qq.com.
